AGERAGE-Induced EMP Release via the NOX-Derived ROS Pathway
Joint Authors
Yan, Xinfeng
Chen, Ying-Hua
Chen, Zhang-Wei
Li, Hong-Mei
Feng, Bo
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs).
This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism.
Methods.
In vitro, cultured human umbilical vein endothelial cells (HUVECs) were incubated with AGEs (200 and 400 μg/ml) for 24 hours with or without pretreatment with anti-RAGE antibody, NOX inhibitor, or ROS scavenger.
The number of CD31-positive EMPs was assessed by flow cytometry.
Results.
The number of EMPs was significantly increased in HUVECs stimulated by AGEs in a dose-dependent manner.
In addition, receptors for AGEs (RAGE), NAD(P)H oxidase (NOX), and reactive oxygen species (ROS) were increased by AGEs as compared to the control group.
These changes could be reversed when HUVECs were pretreated with anti-RAGE antibody.
Moreover, inhibition of NOX as well as antioxidant treatment reduced the release of EMPs induced by AGEs.
Conclusion.
Our study suggested that AGEs increased EMP generation, which was mediated by RAGE signaling through NOX-derived ROS.
American Psychological Association (APA)
Chen, Ying-Hua& Chen, Zhang-Wei& Li, Hong-Mei& Yan, Xinfeng& Feng, Bo. 2018. AGERAGE-Induced EMP Release via the NOX-Derived ROS Pathway. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1183810
Modern Language Association (MLA)
Chen, Ying-Hua…[et al.]. AGERAGE-Induced EMP Release via the NOX-Derived ROS Pathway. Journal of Diabetes Research No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1183810
American Medical Association (AMA)
Chen, Ying-Hua& Chen, Zhang-Wei& Li, Hong-Mei& Yan, Xinfeng& Feng, Bo. AGERAGE-Induced EMP Release via the NOX-Derived ROS Pathway. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1183810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183810