Effects of High Glucose on the Expression of LAMA1 and Biological Behavior of Choroid Retinal Endothelial Cells
Joint Authors
Gao, Qianying
Song, Guangwei
Lin, Da
Bao, Licheng
Jiang, Qi
Zhang, Yinan
Zheng, Haihua
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Hyperglycemia is one of the main causes of proliferative diabetic retinopathy (PDR) characterized by thickening of the vascular basement membrane.
Laminin alpha 1 (LAMA1) is a primary component of laminin, a major protein constituent of the basement membrane.
In this study, we investigated the role of LAMA1 in the development of PDR.
Retinal choroidal vascular endothelial cells (RF/6A line) were exposed to glucose at different concentrations (5 mM, 15 mM, 25 mM, and 35 mM) and analyzed for cell growth, migration, proliferation, and adhesion.
LAMA1 expression was examined 24 and 48 h following glucose treatment using Western blotting, RT-PCR, and immunofluorescence.
The results showed that the proliferation, migration, and adhesion of RF/6A cells were increased by high glucose, whereas LAMA1 expression was slightly higher at 15 mM but decreased at 25 mM and 35 mM glucose compared to control.
Thus, the changes in the biological behavior of high glucose-exposed retinal vascular endothelial cells correspond to variations in LAMA1 expression, indicating a possibility for LAMA1 involvement in PDR development.
Our findings suggest that LAMA1 may play a role in PDR and, thus, may serve as a potential target for DR diagnosis and/or treatment.
American Psychological Association (APA)
Song, Guangwei& Lin, Da& Bao, Licheng& Jiang, Qi& Zhang, Yinan& Zheng, Haihua…[et al.]. 2018. Effects of High Glucose on the Expression of LAMA1 and Biological Behavior of Choroid Retinal Endothelial Cells. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1183875
Modern Language Association (MLA)
Song, Guangwei…[et al.]. Effects of High Glucose on the Expression of LAMA1 and Biological Behavior of Choroid Retinal Endothelial Cells. Journal of Diabetes Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1183875
American Medical Association (AMA)
Song, Guangwei& Lin, Da& Bao, Licheng& Jiang, Qi& Zhang, Yinan& Zheng, Haihua…[et al.]. Effects of High Glucose on the Expression of LAMA1 and Biological Behavior of Choroid Retinal Endothelial Cells. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1183875
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183875