Oral Administration of the Endocannabinoid Anandamide during Lactation: Effects on Hypothalamic Cannabinoid Type 1 Receptor and Food Intake in Adult Mice
Joint Authors
Castillo, Valeska
Aguirre, Carolina
Llanos, Miguel
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-20
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
We have previously shown that administration of the endocannabinoid anandamide (AEA) during lactation leads to overweight, increased body fat accumulation, and insulin resistance in adult mice.
This study was designed to elucidate if these effects are due to increased food intake, stimulated by an augmented abundance and binding ability of the hypothalamic cannabinoid type 1 receptor (CB1R).
With this aim, male mice pups were treated with a daily oral dose of AEA during lactation.
Adult mice were also treated with a single oral dose of AEA, to evaluate acute food intake during 4 h.
At 21 and 160 days, CB1R protein abundance was calculated by western blot analysis.
Capacity of hypothalamic membranes to specifically bind the radioligand 3[H]-CP55.940 was also measured.
Western blots showed a 72% increase in CB1R abundance in AEA-treated 21-day-old mice, without differences in adult mice.
Additionally, specific binding of 3[H]-CP55.940 to hypothalamic membranes from adult mice was significantly lower in those mice treated with AEA during lactation.
Moreover, AEA did not stimulate acute food intake in both, AEA-treated and control mice.
Results suggest that metabolic alterations found in adult mice because of AEA treatment during lactation are not associated with hypothalamic CB1R.
American Psychological Association (APA)
Aguirre, Carolina& Castillo, Valeska& Llanos, Miguel. 2017. Oral Administration of the Endocannabinoid Anandamide during Lactation: Effects on Hypothalamic Cannabinoid Type 1 Receptor and Food Intake in Adult Mice. Journal of Nutrition and Metabolism،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1183925
Modern Language Association (MLA)
Aguirre, Carolina…[et al.]. Oral Administration of the Endocannabinoid Anandamide during Lactation: Effects on Hypothalamic Cannabinoid Type 1 Receptor and Food Intake in Adult Mice. Journal of Nutrition and Metabolism No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1183925
American Medical Association (AMA)
Aguirre, Carolina& Castillo, Valeska& Llanos, Miguel. Oral Administration of the Endocannabinoid Anandamide during Lactation: Effects on Hypothalamic Cannabinoid Type 1 Receptor and Food Intake in Adult Mice. Journal of Nutrition and Metabolism. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1183925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183925