Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii
Joint Authors
Yang, Qiya
Solairaj, Dhanasekaran
Apaliya, Maurice Tibiru
Abdelhai, Mandour
Zhu, Marui
Yan, Yuan
Zhang, Hongyin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-11
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Antagonistic yeasts can inhibit fungal growth.
In our previous research, Meyerozyma guilliermondii, one of the antagonistic yeasts, exhibited antagonistic activity against Penicillium expansum.
However, the mechanisms, especially the molecular mechanisms of inhibiting activity of M.
guilliermondii, are not clear.
In this study, the protein expression profile and transcriptome characterization of P.
expansum induced by M.
guilliermondii were investigated.
In P.
expansum induced by M.
guilliermondii, 66 proteins were identified as differentially expressed, among them six proteins were upregulated and 60 proteins were downregulated, which were associated with oxidative phosphorylation, ATP synthesis, basal metabolism, and response regulation.
Simultaneously, a transcriptomic approach based on RNA-Seq was applied to annotate the genome of P.
expansum and then studied the changes of gene expression in P.
expansum treated with M.
guilliermondii.
The results showed that differentially expressed genes such as HEAT, Phosphoesterase, Polyketide synthase, ATPase, and Ras-association were significantly downregulated, in contrast to Cytochromes P450, Phosphatidate cytidylyltransferase, and Glutathione S-transferase, which were significantly upregulated.
Interestingly, the downregulated differentially expressed proteins and genes have a corresponding relationship; these results revealed that these proteins and genes were important in the growth of P.
expansum treated with M.
guilliermondii.
American Psychological Association (APA)
Yang, Qiya& Solairaj, Dhanasekaran& Apaliya, Maurice Tibiru& Abdelhai, Mandour& Zhu, Marui& Yan, Yuan…[et al.]. 2020. Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1184780
Modern Language Association (MLA)
Yang, Qiya…[et al.]. Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii. Journal of Food Quality No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1184780
American Medical Association (AMA)
Yang, Qiya& Solairaj, Dhanasekaran& Apaliya, Maurice Tibiru& Abdelhai, Mandour& Zhu, Marui& Yan, Yuan…[et al.]. Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1184780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1184780