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A Surgical Cryoprobe for Targeted Transcorneal Freezing and Endothelial Cell Removal
Joint Authors
Kinoshita, Shigeru
Okumura, Naoki
Koizumi, Noriko
Akhbanbetova, Alina
Nakano, Shinichiro
Littlechild, Stacy L.
Young, Robert D.
Zvirgzdina, Madara
Fullwood, Nigel J.
Weston, Ian
Weston, Philip
Quantock, Andrew J.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Purpose.
To examine the effects of transcorneal freezing using a new cryoprobe designed for corneal endothelial surgery.
Methods.
A freezing console employing nitrous oxide as a cryogen was used to cool a series of different cryoprobe tip designs made of silver for high thermal conductivity.
In vitro studies were conducted on 426 porcine corneas, followed by preliminary in vivo investigations on three rabbit corneas.
Results.
The corneal epithelium was destroyed by transcorneal freezing, as expected; however, the epithelial basement membrane remained intact.
Reproducible endothelial damage was optimally achieved using a 3.4 mm diameter cryoprobe with a concave tip profile.
Stromal edema was seen in the pre-Descemet’s area 24 hrs postfreeze injury, but this had been resolved by 10 days postfreeze.
A normal collagen fibril structure was seen 1 month postfreeze, concurrent with endothelial cell repopulation.
Conclusions.
Transcorneal freezing induces transient posterior stromal edema and some residual deep stromal haze but leaves the epithelial basement membrane intact, which is likely to be important for corneal re-epithelialization.
Localized destruction of the endothelial monolayer was achieved in a consistent manner with a 3.4 mm diameter/concave profile cryoprobe and represents a potentially useful approach to remove dysfunctional corneal endothelial cells from corneas with endothelial dysfunction.
American Psychological Association (APA)
Akhbanbetova, Alina& Nakano, Shinichiro& Littlechild, Stacy L.& Young, Robert D.& Zvirgzdina, Madara& Fullwood, Nigel J.…[et al.]. 2017. A Surgical Cryoprobe for Targeted Transcorneal Freezing and Endothelial Cell Removal. Journal of Ophthalmology،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1185046
Modern Language Association (MLA)
Akhbanbetova, Alina…[et al.]. A Surgical Cryoprobe for Targeted Transcorneal Freezing and Endothelial Cell Removal. Journal of Ophthalmology No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1185046
American Medical Association (AMA)
Akhbanbetova, Alina& Nakano, Shinichiro& Littlechild, Stacy L.& Young, Robert D.& Zvirgzdina, Madara& Fullwood, Nigel J.…[et al.]. A Surgical Cryoprobe for Targeted Transcorneal Freezing and Endothelial Cell Removal. Journal of Ophthalmology. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1185046
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1185046