The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment
Joint Authors
Du, Zhiyu
Yan, Pisong
Luo, Qiang
Zhang, Yu
Wang, Menglei
Li, Meixuan
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
To evaluate the safety of high-intensity focused ultrasound keratoplasty as a treatment for presbyopia by examining its effect on the rabbit anterior segment.
Methods.
The right corneas of 36 New Zealand rabbits were treated with HIFU keratoplasty.
The animals were sacrificed at 1, 7, 15, 30, 60, and 90 days after operation.
Collagen type I, MMP-2, and MMP-9 were evaluated using immunohistochemistry.
For the detection of apoptosis, the TUNEL method was applied.
The SOD and MDA levels were analyzed with assay kits.
Results.
Collagen type I, MMP-2, and MMP-9 levels were altered after the operation but returned to normal within 90 days.
The apoptotic index (AI) of the corneal cells decreased from 1 to 30 days gradually.
No apoptosis was observed in the epithelial cells of the lens, and the SOD and MDA levels were normal at any time point.
Conclusion.
After HIFU keratoplasty, the histomorphology of the cornea changed, the corneal collagen type I levels decreased, the corneal MMP-2 and MMP-9 levels increased, and the corneal cells underwent apoptosis for a period of time.
Ninety days after the operation, the levels returned to normal, and the lenses were not affected.
Thus, HIFU presents good biological safety for eyes.
American Psychological Association (APA)
Wang, Menglei& Li, Meixuan& Yan, Pisong& Luo, Qiang& Zhang, Yu& Du, Zhiyu. 2017. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment. Journal of Ophthalmology،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1185088
Modern Language Association (MLA)
Wang, Menglei…[et al.]. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment. Journal of Ophthalmology No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1185088
American Medical Association (AMA)
Wang, Menglei& Li, Meixuan& Yan, Pisong& Luo, Qiang& Zhang, Yu& Du, Zhiyu. The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment. Journal of Ophthalmology. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1185088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1185088