Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits

Abstract EN

Purpose.

To evaluate the effect of the Rho-kinase inhibitor Y-27632 on postoperative inflammation and adhesion following extraocular muscle surgery in rabbits.

Methods.

The superior rectus muscle reinsertion was performed on both eyes of 8 New Zealand white rabbits.

After reinsertion, the rabbits received subconjunctival injections of the Rho-kinase inhibitor and saline on each eye.

To assess acute and late inflammatory changes, Ki-67, CD11β+, and F4/80 were evaluated and the sites of muscle reattachment were evaluated for a postoperative adhesion score and histopathologically for collagen formation.

Results.

F4/80 antibody expression was significantly different in the Rho-kinase inhibitor-injected group at both postoperative day 3 and week 4 (p=0.038, 0.031).

However, Ki-67 and CD11β+ were not different the between two groups.

The difference in the SRM/conjunctiva adhesion score between the two groups was also significant (p=0.034).

Conclusion.

Intraoperative subconjunctival injection of the Rho-kinase inhibitor may be effective for adjunctive management of inflammation and fibrosis in rabbit eyes following extraocular muscle surgery.