Analysis of Aminoglycoside Modifying Enzyme Genes Responsible for High-Level Aminoglycoside Resistance among Enterococcal Isolates
Joint Authors
Shete, Vishal
Kumar, Mahadevan
Grover, Naveen
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Enzymatic modification results in high-level resistance to aminoglycoside (HLAR), which eliminates the synergistic bactericidal effect of combined exposure to a cell wall-active agent and an aminoglycoside.
So aim of the study was to determine prevalence of HLAR enterococcal isolate and to study distribution of aminoglycoside modifying enzyme genes in them.
A total of 100 nonrepeat isolates of enterococci from various clinical samples were analyzed.
As per Clinical and Laboratory Standards Institute guidelines enterococci were screened for HLAR by Kirby-Bauer disc diffusion method.
Minimum inhibitory concentration of all isolates for gentamicin and streptomycin was determined by E-test.
Multiplex polymerase chain reaction (PCR) was carried out for HLAR enterococcal isolates to identify aminoglycoside modifying enzymes genes responsible for resistance.
60% isolates were found to be high-level gentamicin resistant (HLGR) whereas 45% isolates were found to be high-level streptomycin resistant (HLSR).
By multiplex PCR 80% HLGR isolates carried bifunctional aminoglycoside modifying enzyme gene aac(6′)-Ie-aph(2′′)-Ia whereas 18 out of 45 high-level streptomycin resistant, that is, 40%, isolates carried aph(3′)-IIIa.
However, aph(2′′)-Ib, aph(2′′)-Ic, aph(2′′)-Id, and ant(4′)-Ia genes which encode other aminoglycosides modifying enzymes were not detected.
Bifunctional aminoglycoside modifying enzyme gene aac(6′)-Ie-aph(2′′)-Ia is the predominant gene responsible for HLAR.
American Psychological Association (APA)
Shete, Vishal& Grover, Naveen& Kumar, Mahadevan. 2017. Analysis of Aminoglycoside Modifying Enzyme Genes Responsible for High-Level Aminoglycoside Resistance among Enterococcal Isolates. Journal of Pathogens،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1186118
Modern Language Association (MLA)
Shete, Vishal…[et al.]. Analysis of Aminoglycoside Modifying Enzyme Genes Responsible for High-Level Aminoglycoside Resistance among Enterococcal Isolates. Journal of Pathogens No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1186118
American Medical Association (AMA)
Shete, Vishal& Grover, Naveen& Kumar, Mahadevan. Analysis of Aminoglycoside Modifying Enzyme Genes Responsible for High-Level Aminoglycoside Resistance among Enterococcal Isolates. Journal of Pathogens. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1186118
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186118