Identification, Characterization, and Structure of Tm16 from Trichuris muris

Joint Authors

Zhan, Bin
Liu, Zhuyun
Kelleher, Alan
Tabb, Shanii
Wei, Junfei
Pollet, Jeroen
Hotez, Peter J.
Bottazzi, Maria Elena
Asojo, Oluwatoyin A.

Source

Journal of Parasitology Research

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-08-14

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Zoology
Diseases

Abstract EN

Trichuriasis is a disease of poverty for which excretory and secretory (ES) products that induce the protective immunity are being investigated as candidate vaccines antigens.

In this study, ES products of T.

muris and immune sera were produced.

The immune sera recognized more than 20 proteins on a 2D-gel of ES products of T.

muris adult worms.

Tm16 was one of the proteins identified by mass spectrometry.

Tm16 shares 57% sequence identity with Ov16, an immunodominant diagnostic antigen from Onchocerca volvulus.

Recombinant Tm16 with a carboxyl terminal hexahistidine was produced using Pichia pastoris.

Polyclonal antibodies against rTm16 were generated by one-prime and two-boost immunization of three female Balb/c mice with 25 μg of recombinant Tm16 emulsified with ISA720 adjuvant.

These polyclonal antibodies confirmed that Tm16 is localized to the ES products and the soluble fraction of the adult worm.

Additionally, the high-resolution crystal structure of Tm16 was solved by molecular replacement.

Tm16 belongs to the phosphatidylethanolamine-binding-like protein (PEBP1) family and this is the first structure of a PEBP1 from a parasite.

American Psychological Association (APA)

Liu, Zhuyun& Kelleher, Alan& Tabb, Shanii& Wei, Junfei& Pollet, Jeroen& Hotez, Peter J.…[et al.]. 2017. Identification, Characterization, and Structure of Tm16 from Trichuris muris. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1186206

Modern Language Association (MLA)

Liu, Zhuyun…[et al.]. Identification, Characterization, and Structure of Tm16 from Trichuris muris. Journal of Parasitology Research No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1186206

American Medical Association (AMA)

Liu, Zhuyun& Kelleher, Alan& Tabb, Shanii& Wei, Junfei& Pollet, Jeroen& Hotez, Peter J.…[et al.]. Identification, Characterization, and Structure of Tm16 from Trichuris muris. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1186206

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1186206