Molecular Cloning and Characterization of Babesia orientalis Rhoptry Neck 2 BoRON2 Protein
Joint Authors
Malobi, Ngabu
He, Lan
Yu, Long
He, Pei
He, Junwei
Sun, Yali
Huang, Yuan
Zhao, Junlong
Source
Journal of Parasitology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Babesiosis caused by Babesia orientalis is one of the most prevalent infections of water buffalo transmitted by Rhipicephalus haemaphysaloides causing a parasitic and hemolytic disease.
The organelles proteins localized in apical membrane especially rhoptries neck and microneme protein form a complex called moving junction important during invasion process of parasites belonging to apicomplexan group, including Babesia species.
A truncated fragment coding a 936 bps fragment was cloned in pMD-19T and subcloned into pET32 (a)+ expression vector, expressed in E.
coli BL21.
Purified recombinant BoRON2 was used to produce polyclonal antibody against BoRON2.
Here, we identified the full sequence of gene encoding the rhoptry neck 2 protein that we named BoRON2 which is 4035 bp in full-length open reading frame without introns, encoding a polypeptide of 1345 amino acids.
Western blot of rBoRON2 probed with buffalo positive serum analysis revealed a band of around 150 kDa in parasite lysates, suggesting an active involvement during invasion process.
These findings most likely are constructive in perspective of ongoing research focused particularly on water buffalo babesiosis prevention and therapeutics and globally provide new information for genes comparative analysis.
American Psychological Association (APA)
Malobi, Ngabu& He, Lan& Yu, Long& He, Pei& He, Junwei& Sun, Yali…[et al.]. 2017. Molecular Cloning and Characterization of Babesia orientalis Rhoptry Neck 2 BoRON2 Protein. Journal of Parasitology Research،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1186228
Modern Language Association (MLA)
Malobi, Ngabu…[et al.]. Molecular Cloning and Characterization of Babesia orientalis Rhoptry Neck 2 BoRON2 Protein. Journal of Parasitology Research No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1186228
American Medical Association (AMA)
Malobi, Ngabu& He, Lan& Yu, Long& He, Pei& He, Junwei& Sun, Yali…[et al.]. Molecular Cloning and Characterization of Babesia orientalis Rhoptry Neck 2 BoRON2 Protein. Journal of Parasitology Research. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1186228
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186228