Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells
Joint Authors
Ke, Min
Zhu, Xiaolu
Zeng, Wen
Wu, Shengyu
Chen, Xiaomin
Zheng, Tian
Guo, Biechuan
Ren, Jiangbo
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Background.
Endoplasmic reticulum stress (ERS) in the retinal Müller cells is a key factor contributing to the retinal inflammation and vascular leakage in diabetic retinopathy (DR).
This study was to investigate the underlying mechanisms through which the 3 main unfolded protein response (UPR) pathways regulate ERS and to examine the expression levels of vascular endothelial growth factor (VEGF) in Müller cells in vitro.
Methods.
Rat Müller cell lines were stimulated with high glucose to mimic a diabetic environment in vitro.
PKR-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme 1 (IRE1) and activating transcription factor 6 (ATF6) were downregulated or upregulated with shRNA or overexpression plasmids.
The transfected Müller cells were cultivated in high glucose medium for 48 hours.
Expression of glucose-regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), X-box binding protein 1 (XBP1), ATF6, and VEGF was examined with immunofluorescence and western blot.
Results.
Our data indicated that ERS was found in both high glucose and osmotic control groups.
Overexpression or downregulation of UPR pathways effectively increased or reduced the production of GRP78, ATF4, XBP1, ATF6, and VEGF, respectively.
These 3 signaling pathways had similar regulatory effects on VEGF.
Conclusion.
The 3 UPR-mediated inflammatory pathways were dependent on each other.
Inhibition any of these signaling pathways in UPR might be a potential therapeutic target for DR.
American Psychological Association (APA)
Wu, Shengyu& Zhu, Xiaolu& Guo, Biechuan& Zheng, Tian& Ren, Jiangbo& Zeng, Wen…[et al.]. 2019. Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells. Journal of Ophthalmology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1186414
Modern Language Association (MLA)
Wu, Shengyu…[et al.]. Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells. Journal of Ophthalmology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1186414
American Medical Association (AMA)
Wu, Shengyu& Zhu, Xiaolu& Guo, Biechuan& Zheng, Tian& Ren, Jiangbo& Zeng, Wen…[et al.]. Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells. Journal of Ophthalmology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1186414
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186414