Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells

Joint Authors

Ke, Min
Zhu, Xiaolu
Zeng, Wen
Wu, Shengyu
Chen, Xiaomin
Zheng, Tian
Guo, Biechuan
Ren, Jiangbo

Source

Journal of Ophthalmology

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-02-24

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Medicine

Abstract EN

Background.

Endoplasmic reticulum stress (ERS) in the retinal Müller cells is a key factor contributing to the retinal inflammation and vascular leakage in diabetic retinopathy (DR).

This study was to investigate the underlying mechanisms through which the 3 main unfolded protein response (UPR) pathways regulate ERS and to examine the expression levels of vascular endothelial growth factor (VEGF) in Müller cells in vitro.

Methods.

Rat Müller cell lines were stimulated with high glucose to mimic a diabetic environment in vitro.

PKR-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme 1 (IRE1) and activating transcription factor 6 (ATF6) were downregulated or upregulated with shRNA or overexpression plasmids.

The transfected Müller cells were cultivated in high glucose medium for 48 hours.

Expression of glucose-regulated protein 78 (GRP78), activating transcription factor 4 (ATF4), X-box binding protein 1 (XBP1), ATF6, and VEGF was examined with immunofluorescence and western blot.

Results.

Our data indicated that ERS was found in both high glucose and osmotic control groups.

Overexpression or downregulation of UPR pathways effectively increased or reduced the production of GRP78, ATF4, XBP1, ATF6, and VEGF, respectively.

These 3 signaling pathways had similar regulatory effects on VEGF.

Conclusion.

The 3 UPR-mediated inflammatory pathways were dependent on each other.

Inhibition any of these signaling pathways in UPR might be a potential therapeutic target for DR.

American Psychological Association (APA)

Wu, Shengyu& Zhu, Xiaolu& Guo, Biechuan& Zheng, Tian& Ren, Jiangbo& Zeng, Wen…[et al.]. 2019. Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells. Journal of Ophthalmology،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1186414

Modern Language Association (MLA)

Wu, Shengyu…[et al.]. Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells. Journal of Ophthalmology No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1186414

American Medical Association (AMA)

Wu, Shengyu& Zhu, Xiaolu& Guo, Biechuan& Zheng, Tian& Ren, Jiangbo& Zeng, Wen…[et al.]. Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells. Journal of Ophthalmology. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1186414

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1186414