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Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes
Joint Authors
Tyler, K.
Ochaya, Stephen
Franzén, Oscar
Buhwa, Doreen Asiimwe
Foyn, Håvard
Butler, Claire E.
Stove, Svein Isungset
Arnesen, Thomas
Matovu, Enock
Åslund, Lena
Andersson, Björn
Source
Journal of Parasitology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Protein N-terminal acetylation is a co- and posttranslational modification, conserved among eukaryotes.
It determines the functional fate of many proteins including their stability, complex formation, and subcellular localization.
N-terminal acetyltransferases (NATs) transfer an acetyl group to the N-termini of proteins, and the major NATs in yeast and humans are NatA, NatB, and NatC.
In this study, we characterized the Trypanosoma cruzi (T.
cruzi) NatC and NatA protein complexes, each consisting of one catalytic subunit and predicted auxiliary subunits.
The proteins were found to be expressed in the three main life cycle stages of the parasite, formed stable complexes in vivo, and partially cosedimented with the ribosome in agreement with a cotranslational function.
An in vitro acetylation assay clearly demonstrated that the acetylated substrates of the NatC catalytic subunit from T.
cruzi were similar to those of yeast and human NatC, suggesting evolutionary conservation of function.
An RNAi knockdown of the Trypanosoma brucei (T.
brucei) NatC catalytic subunit indicated that reduced NatC-mediated N-terminal acetylation of target proteins reduces parasite growth.
American Psychological Association (APA)
Ochaya, Stephen& Franzén, Oscar& Buhwa, Doreen Asiimwe& Foyn, Håvard& Butler, Claire E.& Stove, Svein Isungset…[et al.]. 2019. Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes. Journal of Parasitology Research،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1186820
Modern Language Association (MLA)
Ochaya, Stephen…[et al.]. Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes. Journal of Parasitology Research No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1186820
American Medical Association (AMA)
Ochaya, Stephen& Franzén, Oscar& Buhwa, Doreen Asiimwe& Foyn, Håvard& Butler, Claire E.& Stove, Svein Isungset…[et al.]. Characterization of Evolutionarily Conserved Trypanosoma cruzi NatC and NatA-N-Terminal Acetyltransferase Complexes. Journal of Parasitology Research. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1186820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186820