miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob

Joint Authors

Tang, Haocheng
Lai, Yin-Yan
Zheng, Jing
Jiang, Hong-Yan
Xu, Geng

Source

Journal of Immunology Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-06-18

Country of Publication

Egypt

No. of Pages

17

Main Subjects

Biology

Abstract EN

Background.

The role of miR-223-3p in dendritic cells (DCs) is unknown.

This study is aimed at investigating the effect of miR-223-3p on the antigen uptake and presentation capacities of DCs and the underlying molecular mechanism.

Methods.

FITC-OVA antigen uptake and cell surface markers in bone marrow-derived DCs (BMDCs) were analyzed by flow cytometry.

BMDCs were transfected with the miR-223-3p mimic or inhibitor.

Cytokine levels were determined by ELISA.

CD4+ T cell differentiation was determined by mixed lymphocyte culture assay.

Results.

OVA treatment significantly downregulated miR-223-3p in BMDCs.

The miR-223-3p mimic significantly inhibited OVA-induced antigen uptake and surface expression of MHC-II on BMDCs (P<0.01).

The miR-223-3p mimic increased TGF-β1 production in OVA-treated DCs (P<0.01).

Mixed lymphocyte reaction showed that the miR-223-3p mimic significantly promoted Treg cell differentiation.

In addition, the miR-223-3p mimic significantly upregulated CD103 in DCs, indicating the promotion of tolerogenic DCs.

The miR-223-3p mimic downregulated Rhob protein in OVA-induced DCs.

Rhob knockdown significantly suppressed the ability of FITC-OVA endocytosis (P<0.01) and surface MHC-II molecule expression (P<0.01) in BMDCs, promoting promoted Treg cell differentiation.

Mannose receptor (MR) knockdown significantly upregulated miR-223-3p, downregulated Rhob protein in OVA-treated DCs, inhibited the FITC-OVA endocytosis and surface MHC-II expression in BMDCs, and promoted Treg cell differentiation (all P<0.01).

Conclusion.

These data suggest that miR-223-3p has an inhibitory effect on the antigen uptake and presentation capacities of BMDCs and promotes Treg cell differentiation, which is, at least partially, through targeting MR signaling and Rhob.

American Psychological Association (APA)

Tang, Haocheng& Lai, Yin-Yan& Zheng, Jing& Jiang, Hong-Yan& Xu, Geng. 2020. miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1186886

Modern Language Association (MLA)

Tang, Haocheng…[et al.]. miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob. Journal of Immunology Research No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1186886

American Medical Association (AMA)

Tang, Haocheng& Lai, Yin-Yan& Zheng, Jing& Jiang, Hong-Yan& Xu, Geng. miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1186886

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1186886