Erythromycin Suppresses the Cigarette Smoke Extract-Exposed Dendritic Cell-Mediated Polarization of CD4+ T Cells into Th17 Cells
Joint Authors
Liu, Jifeng
He, Zhiyi
Zhang, Jianquan
Bai, Jing
Liu, Guangnan
Liang, Yi
Ya, Leilei
Qin, Xianglin
Zhong, Xiao-Ning
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Cigarette smoke is a major effector of chronic obstructive pulmonary disease (COPD), and Th17 cells and dendritic cells (DCs) involve in the pathogenesis of COPD.
Previous studies have demonstrated the anti-inflammatory effects of macrolides.
However, the effects of macrolides on the cigarette smoke extract- (CSE-) induced immune response are unclear.
Accordingly, in this study, we evaluated the effects of erythromycin (EM) on CSE-exposed DCs polarizing naïve CD4+ T cells into Th17 cells.
DCs were generated from bone marrow-derived mononuclear cells isolated from male BALB/c mice and divided into five groups: control DC group, CSE-exposed DC group, CD40-antibody-blocked CSE-exposed DC group, and EM-treated CSE-exposed DC group.
The function of polarizing CD4+ T cells into Th17 cells induced by all four groups of DCs was assayed based on the mixed lymphocyte reaction (MLR) of naïve CD4+ T cells.
CD40 expression in DCs in the CSE-exposed group increased significantly compared with that in the control group (P<0.05).
The Th17 cells in the CSE-exposed DC/MLR group increased significantly compared with those in the control DC/MLR group (P<0.05).
Moreover, Th17 cells in the CD40-blocked CSE-exposed DC/MLR group and EM-treated CSE-exposed DC/MLR group were reduced compared with those in the CSE-exposed DC/MLR group (P<0.05).
Thus, these findings suggested that EM suppressed the CSE-exposed DC-mediated polarization of CD4+ T cells into Th17 cells and that this effect may be mediated through inhibition of the CD40/CD40L pathway.
American Psychological Association (APA)
Liu, Jifeng& Zhong, Xiao-Ning& He, Zhiyi& Zhang, Jianquan& Bai, Jing& Liu, Guangnan…[et al.]. 2020. Erythromycin Suppresses the Cigarette Smoke Extract-Exposed Dendritic Cell-Mediated Polarization of CD4+ T Cells into Th17 Cells. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1186888
Modern Language Association (MLA)
Liu, Jifeng…[et al.]. Erythromycin Suppresses the Cigarette Smoke Extract-Exposed Dendritic Cell-Mediated Polarization of CD4+ T Cells into Th17 Cells. Journal of Immunology Research No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1186888
American Medical Association (AMA)
Liu, Jifeng& Zhong, Xiao-Ning& He, Zhiyi& Zhang, Jianquan& Bai, Jing& Liu, Guangnan…[et al.]. Erythromycin Suppresses the Cigarette Smoke Extract-Exposed Dendritic Cell-Mediated Polarization of CD4+ T Cells into Th17 Cells. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1186888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1186888