M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin

Joint Authors

Ngabire, Daniel
Niyonizigiye, Irvine
Patil, Maheshkumar Prakash
Seong, Yeong-Ae
Seo, Yong Bae
Kim, Gun-Do

Source

Journal of Immunology Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-11-25

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Biology

Abstract EN

Tumor microenvironment components dictate the growth and progression of various cancers.

Tumor-associated macrophages are the most predominant cells in TME and play a major role in cancer invasiveness.

Gastric cancer is one of the most common cancers in Asia, and recently, various cases of resistance to fluorouracil treatment have been reported.

In this study, we investigated the role of alternatively activated macrophages in the resistance of AGS gastric cancer cells to fluorouracil.

THP-1 cells were polarized using recombinant human IL-4, then were cocultured with AGS cells treated with fluorouracil.

Cell viability, Western blot, immunofluorescence, and cell invasion were performed for this investigation.

Our results demonstrated that polarized macrophages initiated the survival of treated AGS cells and induced the resistance in AGS by upregulating the expression of integrin β3, focal adhesion protein (FAK), and cofilin proteins.

These results reveal that integrin β3, focal adhesion protein (FAK), and cofilin proteins are potential targets for the improvement of fluorouracil efficacy in gastric cancer treatment.

American Psychological Association (APA)

Ngabire, Daniel& Niyonizigiye, Irvine& Patil, Maheshkumar Prakash& Seong, Yeong-Ae& Seo, Yong Bae& Kim, Gun-Do. 2020. M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1186924

Modern Language Association (MLA)

Ngabire, Daniel…[et al.]. M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin. Journal of Immunology Research No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1186924

American Medical Association (AMA)

Ngabire, Daniel& Niyonizigiye, Irvine& Patil, Maheshkumar Prakash& Seong, Yeong-Ae& Seo, Yong Bae& Kim, Gun-Do. M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1186924

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1186924