The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice

Joint Authors

Salem, Mohamed L.
El-Naggar, Sabry A.
Mobasher, Maysa A.
Elgharabawy, Rehab M.

Source

Journal of Immunology Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-03-05

Country of Publication

Egypt

No. of Pages

12

Main Subjects

Biology

Abstract EN

One of the activating factors of the cells of the innate immune system is the agonists of toll-like receptors (TLRs).

Our earlier publications detailed how poly(I:C), a TLR3 agonist, elevates the NK cell population and the associated antigen-specific CD8+ T cell responses.

This study involved a single treatment of the B6 mice with poly(I:C) intraperitoneally.

To perform a detailed phenotypic analysis, mononuclear cells were prepared from each of the liver, peripheral blood, and spleen.

These cells were then examined for their NK cell population by flow cytometric analysis following cell staining with indicated antibodies.

The findings of the study showed that the NK cell population of the liver with an NK1.1highCD11bhighCD11chigh B220+Ly6G- phenotype was elevated following the treatment with poly(I:C).

In the absence of CD11b molecule (CR3-/- mice), poly(I:C) can still increase the remained numbers of NK cells with NK1.1+CD11b- and NK1.1+Ly6G- phenotypes in the liver while their numbers in the blood decrease.

After the treatment with anti-AGM1 Ab, which induced depletion of NK1.1+CD11b+ cells and partial depletion of CD3+NK1.1+ and NK1.1+CD11b- cell populations, poly(I:C) normalized the partial decreases in the numbers of NK cells concomitant with increased numbers of NK1.1-CD11b+ cell population in both liver and blood.

Regarding mice with a TLR3-/- phenotype, their injection with poly(I:C) resulted in the partial elevation in the NK cell population as compared to wild-type B6 mice.

To summarise, the TLR3 agonist poly(I:C) results in the elevation of a subset of liver NK cells expressing the two myeloid markers CD11c and CD11b.

The effect of poly(I:C) on NK cells is partially dependent on TLR3 and independent of the presence of CD11b.

American Psychological Association (APA)

Salem, Mohamed L.& El-Naggar, Sabry A.& Mobasher, Maysa A.& Elgharabawy, Rehab M.. 2020. The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1187020

Modern Language Association (MLA)

Salem, Mohamed L.…[et al.]. The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice. Journal of Immunology Research No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1187020

American Medical Association (AMA)

Salem, Mohamed L.& El-Naggar, Sabry A.& Mobasher, Maysa A.& Elgharabawy, Rehab M.. The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1187020

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1187020