Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo
Joint Authors
Tamminen, Kirsi
Vesikari, Timo
Blazevic, Vesna
Heinimäki, Suvi
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-23
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
We have previously shown that rotavirus (RV) inner capsid protein VP6 has an adjuvant effect on norovirus (NoV) virus-like particle- (VLP-) induced immune responses and studied the adjuvant mechanism in immortalized cell lines used as antigen-presenting cells (APCs).
Here, we investigated the uptake and presentation of RV VP6 and NoV GII.4 VLPs by primary bone marrow-derived dendritic cells (BMDCs).
The adjuvant effect of VP6 on GII.4 VLP presentation and NoV-specific immune response induction by BMDC in vivo was also studied.
Intracellular staining demonstrated that BMDCs internalized both antigens, but VP6 more efficiently than NoV VLPs.
Both antigens were processed and presented to antigen-primed T cells, which responded by robust interferon γ secretion.
When GII.4 VLPs and VP6 were mixed in the same pulsing reaction, a subpopulation of the cells had uptaken both antigens.
Furthermore, VP6 copulsing increased GII.4 VLP uptake by 37% and activated BMDCs to secrete 2-5-fold increased levels of interleukin 6 and tumor necrosis factor α compared to VLP pulsing alone.
When in vitro-pulsed BMDCs were transferred to syngeneic BALB/c mice, VP6 improved NoV-specific antibody responses.
The results of this study support the earlier findings of VP6 adjuvant effect in vitro and in vivo.
American Psychological Association (APA)
Tamminen, Kirsi& Heinimäki, Suvi& Vesikari, Timo& Blazevic, Vesna. 2020. Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1187122
Modern Language Association (MLA)
Tamminen, Kirsi…[et al.]. Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo. Journal of Immunology Research No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1187122
American Medical Association (AMA)
Tamminen, Kirsi& Heinimäki, Suvi& Vesikari, Timo& Blazevic, Vesna. Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1187122
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187122