Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine
Joint Authors
Chen, Chumeng
Aldarouish, Mohanad
Li, Qilong
Liu, Xiangzhen
Han, Feng
Liu, Hui
Qian, Qijun
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The success of peptide-based dendritic cell (DC) cancer vaccines mainly depends on the utilized peptides and selection of an appropriate adjuvant.
Herein, we aimed to evoke a broad immune response against multiple epitopes concurrently in the presence of immunoadjuvant.
Three synthetic HLA-A∗0201-restricted peptides were separately linked with HMGB1-derived peptide (SAFFLFCSE, denoted as HB100-108) as immunoadjuvant via double arginine (RR) linker and loaded onto human monocyte-derived DCs.
Peptide uptake was detected by immunofluorescence microscopy and flow cytometry.
The maturation and activation status of pulsed DCs were monitored by detection of the expression of specific markers and released cytokines.
The ability of peptide-pulsed DCs to activate allogeneic T cells has been assessed by a degranulation assay and detection of secreted cytokines.
The lytic activity of effector T cells against cancer cells in vitro was analyzed by a lactate dehydrogenase (LDH) assay.
Results revealed that DCs efficiently take up peptides+HB100-108 and expressed higher levels of surface markers (HLA-ABC, HLA-DR, CD80, CD86, CD83, CD40, and CCR7) and proinflammatory cytokines (IL-6, IFN-γ, TNF-α, and IL-12) than control DCs, free peptide-pulsed DCs, and free HB100-108-pulsed DC groups.
Moreover, peptides+HB100-108/pulsed DCs were capable of activating allogeneic T cells and enhance their lytic activity against a pancreatic cancer cell line (PANC-1) in vitro.
These findings suggest that antigenic peptides covalently linked with HB100-108/pulsed DCs could be a promising strategy to improve the current DC-based cancer vaccines.
American Psychological Association (APA)
Chen, Chumeng& Aldarouish, Mohanad& Li, Qilong& Liu, Xiangzhen& Han, Feng& Liu, Hui…[et al.]. 2020. Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1187168
Modern Language Association (MLA)
Chen, Chumeng…[et al.]. Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine. Journal of Immunology Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1187168
American Medical Association (AMA)
Chen, Chumeng& Aldarouish, Mohanad& Li, Qilong& Liu, Xiangzhen& Han, Feng& Liu, Hui…[et al.]. Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1187168
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187168