Effects of Epstein-Barr Virus Infection on CD19+ B Lymphocytes in Patients with Immunorelated Pancytopenia
Joint Authors
Ding, Kai
Fu, Rong
Wang, Yihao
Liu, Chunyan
Zhao, Yang
Shao, Zonghong
Yu, Hong
Liu, Hui
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objectives.
To explore effects of Epstein-Barr virus (EBV) infection on CD19+ B lymphocytes in patients with immunorelated pancytopenia (IRP).
Methods.
An enzyme-linked immunosorbent assay (ELISA) in vitro diagnostic kit was used to detect EBV capsid antigen- (CA-) IgG and VCA-IgM antibodies in the serum.
We analyzed the EBV-DNA copies of CD19+ B lymphocyte by using real-time quantitative polymerase chain reaction (RT-qPCR).
CD21, CD23, CD5, CD80, and CD86 receptors on the surfaces of CD19+ B cells were detected by flow cytometry (FCM).
The correlation between these receptors and EBV-DNA copies were evaluated.
Results.
The results revealed that the positive rate of EBVCA-IgM and CD19+ B lymphocyte EBV-DNA copy in the IRP group were significantly higher than those in the control group (P<0.05).
CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects (P<0.05).
Expression levels of the CD21, CD23, CD5, CD80, and CD86 receptors on the surfaces of CD19+ B cells in IRP patients with anti-EBVCA IgM positivity were significantly higher than those in anti-EBVCA IgM negativity IRP patients (P<0.05).
The results revealed that EBV-DNA copy numbers were positively correlated with CD21, CD23, CD5, CD80, and CD86 expression.
Conclusions.
EBV infection may activate CD19+ B lymphocytes and further disrupt bone marrow hematopoiesis in IRP patients.
American Psychological Association (APA)
Zhao, Yang& Wang, Yihao& Liu, Hui& Ding, Kai& Liu, Chunyan& Yu, Hong…[et al.]. 2020. Effects of Epstein-Barr Virus Infection on CD19+ B Lymphocytes in Patients with Immunorelated Pancytopenia. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187179
Modern Language Association (MLA)
Zhao, Yang…[et al.]. Effects of Epstein-Barr Virus Infection on CD19+ B Lymphocytes in Patients with Immunorelated Pancytopenia. Journal of Immunology Research No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1187179
American Medical Association (AMA)
Zhao, Yang& Wang, Yihao& Liu, Hui& Ding, Kai& Liu, Chunyan& Yu, Hong…[et al.]. Effects of Epstein-Barr Virus Infection on CD19+ B Lymphocytes in Patients with Immunorelated Pancytopenia. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1187179
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187179