Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1
Joint Authors
Li, Yuanyuan
Song, Yueqi
Zhu, Leqing
Wang, Xiao
Richers, Brittany
Leung, Donald Y. M.
Bin, Lianghua
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Type I interferon kappa (IFNκ) is selectively expressed in human keratinocytes.
Herpes simplex virus-1 (HSV-1) is a human pathogen that infects keratinocytes and causes lytic skin lesions.
Whether IFNκ plays a role in keratinocyte host defense against HSV-1 has not been investigated.
In this study, we found that IFNκ mRNA expression was induced by addition of recombinant IFNκ and poly (I:C); and its expression level was significantly greater than IFNa2, IFNb1, and IFNL1 in both undifferentiated and differentiated normal human epidermal keratinocytes (NHEKs) under resting and stimulation conditions.
Although IFNe was expressed at a relatively higher level than other IFNs in resting undifferentiated NHEK, its expression level did not change after stimulation with recombinant IFNκ and poly (I:C).
HSV-1 infection inhibited gene expression of IFNκ and IFNe in NHEK.
Silencing IFNκ in NHEK led to significantly enhanced HSV-1 replication in both undifferentiated and differentiated NHEK compared to scrambled siRNA-transfected cells, while the addition of recombinant IFNκ significantly reduced HSV-1 replication in NHEK.
In addition, we found that IFNκ did not regulate protein expression of NHEK differentiation markers.
Our results demonstrate that IFNκ is the dominant type of IFNs in keratinocytes and it has an important function for keratinocytes to combat HSV-1 infection.
American Psychological Association (APA)
Li, Yuanyuan& Song, Yueqi& Zhu, Leqing& Wang, Xiao& Richers, Brittany& Leung, Donald Y. M.…[et al.]. 2020. Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1187263
Modern Language Association (MLA)
Li, Yuanyuan…[et al.]. Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1. Journal of Immunology Research No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1187263
American Medical Association (AMA)
Li, Yuanyuan& Song, Yueqi& Zhu, Leqing& Wang, Xiao& Richers, Brittany& Leung, Donald Y. M.…[et al.]. Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1187263
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187263