Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17Treg Balance toward Antitumor Th17 Cells
Joint Authors
Rezalotfi, Alaleh
Solgi, Ghasem
Ebrahimi, Marzieh
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Gastrosphere, an enriched cellular population with stem-like properties believed to be responsible for an escape from immune-mediated destruction.
Th17 and Treg cells play a major role in gastric cancer; however, their interaction with gastrospheres remained elusive.
Method.
Peripheral blood mononuclear cells were isolated from healthy donors and were cultured with conditioned media of MKN-45 (parental) cells as well as gastrospheres’ conditioned media in the context of mixed lymphocyte reaction and in the presence of anti-CD3/CD28 beads.
The proliferation was evaluated using CFSE staining; the percentages of CD4+CD25+FoxP3+ Treg and CD4+IL-17+ Th17 cells and IFN-γ+cells and the production of IL-17, TGF-β, and IL-10 were assessed by flow cytometry and ELISA, respectively.
Finally, the cytotoxic potential of induced immune cells was measured by examining the secretion of lactate dehydrogenase from target cells.
Results.
The results revealed a decreased expansion of PBMCs postexposure to gastrospheres’ conditioned medium which was concomitant with an increased percentage of Th17 and an enhanced Th17 to Treg ratio.
The conditioned media of gastrospheres enhanced the secretion of IL-10 and IL-17 and decreased TGF-β.
Interestingly, immune cells induced by gastrospheres showed significant cytotoxicity in terms of producing IFN-γ and death induction in target cells.
All these changes were related to the upregulation of IL-6, IL-10, and IL-22 in gastrospheres compared to parental cells.
Conclusion.
Our study showed that the condition media of gastrospheres can potentially induce Th17 with increasing in their cytotoxic effect.
Based on our knowledge, the present study is the first study that emphasizes the role of gastrospheres in the induction of antitumor Th17 cells.
However, it should be confirmed with complementary studies in vivo.
American Psychological Association (APA)
Rezalotfi, Alaleh& Solgi, Ghasem& Ebrahimi, Marzieh. 2020. Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17Treg Balance toward Antitumor Th17 Cells. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1187361
Modern Language Association (MLA)
Rezalotfi, Alaleh…[et al.]. Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17Treg Balance toward Antitumor Th17 Cells. Journal of Immunology Research No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1187361
American Medical Association (AMA)
Rezalotfi, Alaleh& Solgi, Ghasem& Ebrahimi, Marzieh. Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17Treg Balance toward Antitumor Th17 Cells. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1187361
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187361