Multifeature Quantification of Nuclear Properties from Images of H&E-Stained Biopsy Material for Investigating Changes in Nuclear Structure with Advancing CIN Grade
Joint Authors
Kostopoulos, S.
Ravazoula, Panagiota
Sakelaropoulos, George
Konstandinou, Christos
Michail, George
Lavdas, Eleftherios
Kalatzis, Ioannis
Glotsos, D.
Cavouras, D.
Source
Journal of Healthcare Engineering
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Cervical dysplasia is a precancerous condition, and if left untreated, it may lead to cervical cancer, which is the second most common cancer in women.
The purpose of this study was to investigate differences in nuclear properties of the H&E-stained biopsy material between low CIN and high CIN cases and associate those properties with the CIN grade.
Methods.
The clinical material comprised hematoxylin and eosin- (H&E-) stained biopsy specimens from lesions of 44 patients diagnosed with cervical intraepithelial neoplasia (CIN).
Four or five nonoverlapping microscopy images were digitized from each patient’s H&E specimens, from regions indicated by the expert physician.
Sixty-three textural and morphological nuclear features were generated for each patient’s images.
The Wilcoxon statistical test and the point biserial correlation were used to estimate each feature’s discriminatory power between low CIN and high CIN cases and its correlation with the advancing CIN grade, respectively.
Results.
Statistical analysis showed 19 features that quantify nuclear shape, size, and texture and sustain statistically significant differences between low CIN and high CIN cases.
These findings revealed that nuclei in high CIN cases, as compared to nuclei in low CIN cases, have more irregular shape, are larger in size, are coarser in texture, contain higher edges, have higher local contrast, are more inhomogeneous, and comprise structures of different intensities.
Conclusion.
A systematic statistical analysis of nucleus features, quantified from the H&E-stained biopsy material, showed that there are significant differences in the shape, size, and texture of nuclei between low CIN and high CIN cases.
American Psychological Association (APA)
Konstandinou, Christos& Glotsos, D.& Kostopoulos, S.& Kalatzis, Ioannis& Ravazoula, Panagiota& Michail, George…[et al.]. 2018. Multifeature Quantification of Nuclear Properties from Images of H&E-Stained Biopsy Material for Investigating Changes in Nuclear Structure with Advancing CIN Grade. Journal of Healthcare Engineering،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1187494
Modern Language Association (MLA)
Konstandinou, Christos…[et al.]. Multifeature Quantification of Nuclear Properties from Images of H&E-Stained Biopsy Material for Investigating Changes in Nuclear Structure with Advancing CIN Grade. Journal of Healthcare Engineering No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1187494
American Medical Association (AMA)
Konstandinou, Christos& Glotsos, D.& Kostopoulos, S.& Kalatzis, Ioannis& Ravazoula, Panagiota& Michail, George…[et al.]. Multifeature Quantification of Nuclear Properties from Images of H&E-Stained Biopsy Material for Investigating Changes in Nuclear Structure with Advancing CIN Grade. Journal of Healthcare Engineering. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1187494
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187494