Human Bone Marrow Mesenchymal StemStromal Cells Exposed to an Inflammatory Environment Increase the Expression of ICAM-1 and Release Microvesicles Enriched in This Adhesive Molecule: Analysis of the Participation of TNF-α and IFN-γ
Joint Authors
Arriaga-Pizano, Lourdes
Montesinos, Juan J.
López-García, Lucero
Cortés-Morales, Víctor A.
Valle-Ríos, Ricardo
Fajardo-Orduña, Guadalupe R.
Castro-Manrreza, Marta E.
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-01
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Bone marrow mesenchymal stem/stromal cells (BM-MSCs) have immunoregulatory capacity; therefore, they have been used in different clinical protocols in which it is necessary to decrease the immune response.
This capacity is mainly regulated by TNF-α and IFN-γ, and it has been observed that cell-cell contact, mainly mediated by ICAM-1, is important for MSCs to carry out efficient immunoregulation.
Therefore, in the present work, we analyzed the effect of TNF-α alone or in combination with IFN-γ on the expression of ICAM-1.
Besides, given the importance of cell contact in the immunoregulatory function of MSCs, we analyzed whether these cells release ICAM-1+ microvesicles (MVs).
Our results show for the first time that TNF-α is capable of increasing the early expression of ICAM-1 in human BM-MSCs.
Also, we observed that TNF-α and IFN-γ have a synergistic effect on the increase in the expression of ICAM-1.
Furthermore, we found that BM-MSCs exposed to an inflammatory environment release MVs enriched in ICAM-1 (MVs-ICAM-1high).
The knowledge generated in this study will contribute to the improvement of in vitro conditioning protocols that favor the therapeutic effect of these cells or their products.
American Psychological Association (APA)
Montesinos, Juan J.& López-García, Lucero& Cortés-Morales, Víctor A.& Arriaga-Pizano, Lourdes& Valle-Ríos, Ricardo& Fajardo-Orduña, Guadalupe R.…[et al.]. 2020. Human Bone Marrow Mesenchymal StemStromal Cells Exposed to an Inflammatory Environment Increase the Expression of ICAM-1 and Release Microvesicles Enriched in This Adhesive Molecule: Analysis of the Participation of TNF-α and IFN-γ. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1187621
Modern Language Association (MLA)
Montesinos, Juan J.…[et al.]. Human Bone Marrow Mesenchymal StemStromal Cells Exposed to an Inflammatory Environment Increase the Expression of ICAM-1 and Release Microvesicles Enriched in This Adhesive Molecule: Analysis of the Participation of TNF-α and IFN-γ. Journal of Immunology Research No. 2020 (2020), pp.1-17.
https://search.emarefa.net/detail/BIM-1187621
American Medical Association (AMA)
Montesinos, Juan J.& López-García, Lucero& Cortés-Morales, Víctor A.& Arriaga-Pizano, Lourdes& Valle-Ríos, Ricardo& Fajardo-Orduña, Guadalupe R.…[et al.]. Human Bone Marrow Mesenchymal StemStromal Cells Exposed to an Inflammatory Environment Increase the Expression of ICAM-1 and Release Microvesicles Enriched in This Adhesive Molecule: Analysis of the Participation of TNF-α and IFN-γ. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-17.
https://search.emarefa.net/detail/BIM-1187621
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187621