Leishmania mexicana: Novel Insights of Immune Modulation through Amastigote Exosomes
Joint Authors
Soto-Serna, Laura Enedina
Diupotex, Mariana
Zamora-Chimal, Jaime
Ruiz-Remigio, Adriana
Delgado-Domínguez, José
Cervantes-Sarabia, Rocely Buenaventura
Méndez-Bernal, Adriana
Escalona-Montaño, Alma Reyna
Aguirre-García, María Magdalena
Becker, Ingeborg
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Exosomes are extracellular microvesicles of endosomal origin (multivesicular bodies, MVBs) constitutively released by eukaryotic cells by fusion of MVBs to the plasma membrane.
The exosomes from Leishmania parasites contain an array of parasite molecules such as virulence factors and survival messengers, capable of modulating the host immune response and thereby favoring the infection of the host.
We here show that exosomes of L.
mexicana amastigotes (aExo) contain the virulence proteins gp63 and PP2C.
The incubation of aExo with bone marrow-derived macrophages (BMMs) infected with L.
mexicana led to their internalization and were found to colocalize with the cellular tetraspanin CD63.
Furthermore, aExo inhibited nitric oxide production of infected BMMs, permitting enhanced intracellular parasite survival.
Expressions of antigen-presenting (major histocompatibility complex class I, MHC-I, and CD1d) and costimulatory (CD86 and PD-L1) molecules were modulated in a dose-dependent fashion.
Whereas MHC-I, CD86 and PD-L1 expressions were diminished by exosomes, CD1d was enhanced.
We conclude that aExo of L.
mexicana are capable of decreasing microbicidal mechanisms of infected macrophages by inhibiting nitric oxide production, thereby enabling parasite survival.
They also hamper the cellular immune response by diminishing MHC-I and CD86 on an important antigen-presenting cell, which potentially interferes with CD8 T cell activation.
The enhanced CD1d expression in combination with reduction of PD-L1 on BMMs point to a potential shift of the activation route towards lipid presentations, yet the effectivity of this immune activation is not evident, since in the absence of costimulatory molecules, cellular anergy and tolerance would be expected.
American Psychological Association (APA)
Soto-Serna, Laura Enedina& Diupotex, Mariana& Zamora-Chimal, Jaime& Ruiz-Remigio, Adriana& Delgado-Domínguez, José& Cervantes-Sarabia, Rocely Buenaventura…[et al.]. 2020. Leishmania mexicana: Novel Insights of Immune Modulation through Amastigote Exosomes. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1187642
Modern Language Association (MLA)
Soto-Serna, Laura Enedina…[et al.]. Leishmania mexicana: Novel Insights of Immune Modulation through Amastigote Exosomes. Journal of Immunology Research No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1187642
American Medical Association (AMA)
Soto-Serna, Laura Enedina& Diupotex, Mariana& Zamora-Chimal, Jaime& Ruiz-Remigio, Adriana& Delgado-Domínguez, José& Cervantes-Sarabia, Rocely Buenaventura…[et al.]. Leishmania mexicana: Novel Insights of Immune Modulation through Amastigote Exosomes. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1187642
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187642