Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device
Joint Authors
Ma, Shou-Yu
Chiang, Yu-Cheng
Hsu, Chia-Hsien
Hsu, Chin-Chi
Chao, An-Chong
Chen, Jyh Jian
Lin, Yung-Sheng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In this study, we integrated genetic detection for polymerase chain reaction (PCR) with microfluidics technology for the detection of peanut DNA.
A cross-junction microchannel was used to induce emulsion droplets of water in oil for PCR on a chip.
Compared with the single-phase flow, the emulsion droplet flow exhibited a 7.24% lower evaporation amount and prevented air bubble generation.
PCR results of the droplet microfluidic PCR chip for peanut DNA fragment detection was verified by comparison with a commercial PCR thermal cycler and increased fluorescence intensity in SYBR Green reagent-based PCR.
Moreover, PCR on the microfluidic PCR chip was successful for sesame, Salmonella spp., and Staphylococcus aureus.
The droplet microfluidic PCR device developed in this study can be applied for peanut detection in the context of food allergy.
American Psychological Association (APA)
Ma, Shou-Yu& Chiang, Yu-Cheng& Hsu, Chia-Hsien& Chen, Jyh Jian& Hsu, Chin-Chi& Chao, An-Chong…[et al.]. 2019. Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device. Journal of Sensors،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1187702
Modern Language Association (MLA)
Ma, Shou-Yu…[et al.]. Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device. Journal of Sensors No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1187702
American Medical Association (AMA)
Ma, Shou-Yu& Chiang, Yu-Cheng& Hsu, Chia-Hsien& Chen, Jyh Jian& Hsu, Chin-Chi& Chao, An-Chong…[et al.]. Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device. Journal of Sensors. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1187702
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187702