Characterization and Significance of Monocytes in Acute Stanford Type B Aortic Dissection
Joint Authors
Dou, Huan
Liu, Zhao
Lu, Li
Tong, Yuanhao
Wang, Wenwen
Hou, Yayi
Source
Journal of Immunology Research
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-15
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Acute aortic dissection (AAD) is one of the most common fatal diseases noted in vascular surgery.
Human monocytes circulate in dynamic equilibrium and display a considerable heterogeneity.
However, the role of monocytes in AAD remains elusive.
In our recent study, we firstly obtained blood samples from 22 patients with Stanford type B AAD and 44 age-, sex-, and comorbidity-matched control subjects.
And the monocyte proportions were evaluated by flow cytometry.
Results showed that the percentage of total CD14+ monocytes in the blood samples of Stanford AAD patients was increased significantly compared with that of normal volunteers (P<0.0005), and the absolute numbers of CD14brightCD16+ and CD14brightCD16- monocytes both increased significantly regardless of the percentage of PBMC or CD14+ cells, while CD14dimCD16+ monocytes displayed the opposite tendency.
However, the percentage of CD14+ cells and its three subsets demonstrated no correlation with D-dimer (DD) and C-reactive protein (CRP).
Then, blood mononuclear cell (PBMC) samples were collected by Ficoll density gradient centrifugation, followed with CD14+ magnetic bead sorting.
After the purity of CD14+ cells was validated over 90%, AAD-related genes were concentrated in CD14+ monocytes.
There were no significant differences observed with regard to the mRNA expression levels of MMP1 (P=0.0946), MMP2 (P=0.3941), MMP9 (P=0.2919), IL-6 (P=0.4223), and IL-10 (P=0.3375) of the CD14+ monocytes in Stanford type B AAD patients compared with those of normal volunteers.
The expression levels of IL-17 (P<0.05) was higher in Stanford type B AAD patients, while the expression levels of TIMP1(P<0.05), TIMP2(P<0.01), TGF-β1 (P<0.01), SMAD3 (P<0.01), ACTA2 (P<0.001), and ADAMTS-1 (P<0.001) decreased.
The data suggested that monocytes might play an important role in the development of Stanford type B AAD.
Understanding of the production, differentiation, and function of monocyte subsets might dictate future therapeutic avenues for Stanford type B AAD treatment and can aid the identification of novel biomarkers or potential therapeutic targets for decreasing inflammation in AAD.
American Psychological Association (APA)
Lu, Li& Tong, Yuanhao& Wang, Wenwen& Hou, Yayi& Dou, Huan& Liu, Zhao. 2020. Characterization and Significance of Monocytes in Acute Stanford Type B Aortic Dissection. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1187718
Modern Language Association (MLA)
Lu, Li…[et al.]. Characterization and Significance of Monocytes in Acute Stanford Type B Aortic Dissection. Journal of Immunology Research No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1187718
American Medical Association (AMA)
Lu, Li& Tong, Yuanhao& Wang, Wenwen& Hou, Yayi& Dou, Huan& Liu, Zhao. Characterization and Significance of Monocytes in Acute Stanford Type B Aortic Dissection. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1187718
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187718