Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy
Joint Authors
Minamikawa, Takeo
Murakami, Yoshinori
Matsumura, Naokazu
Niioka, Hirohiko
Fukushima, Shuichiro
Araki, Tsutomu
Hashimoto, Mamoru
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
In this study, we investigated photo-induced damage to living cells during single- and multifocus excitations for coherent anti-Stokes Raman scattering (CARS) imaging.
A near-infrared pulsed laser (709 nm) was used to induce cell damage.
We compared the photo-induced cell damage in the single- and the multifocus excitation schemes with the condition to obtain the same CARS signal in the same frame rate.
For the evaluation of cell viability, we employed 4′,6-diamidino-2-phenylindole (DAPI) fluorophores that predominantly stained the damaged cells.
One- and two-photon fluorescence of DAPI fluorophores were, respectively, excited by an ultraviolet light source and the same near-infrared light source and were monitored to evaluate the cell viability during near-infrared pulsed laser irradiation.
We found lower uptake of DAPI fluorophores into HeLa cells during the multifocus excitation compared with the single-focus excitation scheme in both the one- and the two-photon fluorescence examinations.
This indicates a reduction of photo-induced cell damage in the multifocus excitation.
Our findings suggested that the multifocus excitation scheme is expected to be suitable for CARS microscopy in terms of minimal invasiveness.
American Psychological Association (APA)
Minamikawa, Takeo& Murakami, Yoshinori& Matsumura, Naokazu& Niioka, Hirohiko& Fukushima, Shuichiro& Araki, Tsutomu…[et al.]. 2017. Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Spectroscopy،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1187830
Modern Language Association (MLA)
Minamikawa, Takeo…[et al.]. Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Spectroscopy No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1187830
American Medical Association (AMA)
Minamikawa, Takeo& Murakami, Yoshinori& Matsumura, Naokazu& Niioka, Hirohiko& Fukushima, Shuichiro& Araki, Tsutomu…[et al.]. Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy. Journal of Spectroscopy. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1187830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187830