Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD
Joint Authors
Ma, Choong Je
Ryu, Gahee
Yang, Woo Seung
Weon, Jin Bae
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Nelumbo nucifera has a variety of biological activities.
So it was importantly used as various herbal medicines since traditional times.
A simple, fast, and sensitive high-performance liquid chromatographic (HPLC) method was developed in this study for efficient quality control of N.
nucifera.
Four different compounds, including neferine, 1,2,3,4-tetrahydro-1-[(4-hydroxyphenyl) methyl]-2-methyl-7-isoquinolinol, 1-hydroxy-2-methylpropene, and 3-(prop-1-enyl)benzene-1,2,4,5-tetrol, were simultaneously determined.
The four compounds were isolated through a Dionex C18 column by gradient elution with 0.1% TFA-water and methanol.
The flow rate was 1.0 mL/min, and the wavelength was detected at 205, 254, 280, and 330 nm.
The chromatograms were acquired at 205 nm.
The four compounds showed good linear relationships (r2>0.96) over five different concentrations, and an average recovery of the method ranged from 96.27% to 108.78%.
Through the analysis validation test and application of the method, the optimized conditions verified that it is efficient to isolate the compounds of N.
nucifera seed embryos.
American Psychological Association (APA)
Ryu, Gahee& Weon, Jin Bae& Yang, Woo Seung& Ma, Choong Je. 2017. Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD. Journal of Spectroscopy،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1187840
Modern Language Association (MLA)
Ryu, Gahee…[et al.]. Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD. Journal of Spectroscopy No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1187840
American Medical Association (AMA)
Ryu, Gahee& Weon, Jin Bae& Yang, Woo Seung& Ma, Choong Je. Simultaneous Determination of Four Compounds in a Nelumbo nucifera Seed Embryo by HPLC-DAD. Journal of Spectroscopy. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1187840
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187840