Application of Near-Infrared Spectroscopy to Quantitatively Determine Relative Content of Puccnia striiformis f. sp. tritici DNA in Wheat Leaves in Incubation Period
Joint Authors
Cheng, Pei
Li, Xiaolong
Qin, Feng
Zhao, Longlian
Li, Junhui
Ma, Zhanhong
Wang, Haiguang
Gu, Yilin
Zhao, Yaqiong
Pan, Yang
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-06
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Stripe rust caused by Puccinia striiformis f.
sp.
tritici (Pst) is a devastating wheat disease worldwide.
Potential application of near-infrared spectroscopy (NIRS) in detection of pathogen amounts in latently Pst-infected wheat leaves was investigated for disease prediction and control.
A total of 300 near-infrared spectra were acquired from the Pst-infected leaf samples in an incubation period, and relative contents of Pst DNA in the samples were obtained using duplex TaqMan real-time PCR arrays.
Determination models of the relative contents of Pst DNA in the samples were built using quantitative partial least squares (QPLS), support vector regression (SVR), and a method integrated with QPLS and SVR.
The results showed that the kQPLS-SVR model built with a ratio of training set to testing set equal to 3 : 1 based on the original spectra, when the number of the randomly selected wavelength points was 700, the number of principal components was 8, and the number of the built QPLS models was 5, was the best.
The results indicated that quantitative detection of Pst DNA in leaves in the incubation period could be implemented using NIRS.
A novel method for determination of latent infection levels of Pst and early detection of stripe rust was provided.
American Psychological Association (APA)
Zhao, Yaqiong& Gu, Yilin& Qin, Feng& Li, Xiaolong& Ma, Zhanhong& Zhao, Longlian…[et al.]. 2017. Application of Near-Infrared Spectroscopy to Quantitatively Determine Relative Content of Puccnia striiformis f. sp. tritici DNA in Wheat Leaves in Incubation Period. Journal of Spectroscopy،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1187878
Modern Language Association (MLA)
Zhao, Yaqiong…[et al.]. Application of Near-Infrared Spectroscopy to Quantitatively Determine Relative Content of Puccnia striiformis f. sp. tritici DNA in Wheat Leaves in Incubation Period. Journal of Spectroscopy No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1187878
American Medical Association (AMA)
Zhao, Yaqiong& Gu, Yilin& Qin, Feng& Li, Xiaolong& Ma, Zhanhong& Zhao, Longlian…[et al.]. Application of Near-Infrared Spectroscopy to Quantitatively Determine Relative Content of Puccnia striiformis f. sp. tritici DNA in Wheat Leaves in Incubation Period. Journal of Spectroscopy. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1187878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187878