Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae)
Joint Authors
Caldeira, Roberta Lima
Carvalho, Omar dos Santos
Jannotti-Passos, Liana Konovaloff
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of S.
mansoni DNA in (1) Brazilian intermediate hosts (Biomphalaria glabrata, B.
straminea, and B.
tenagophila) with patent S.
mansoni infections, (2) B.
glabrata snails with prepatent S.
mansoni infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species.
The assays showed high sensitivity and specificity and could detect S.
mansoni DNA when one positive snail was included in a pool of 1,000 negative specimens of Biomphalaria.
These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of S.
mansoni in pooled samples of field-collected Biomphalaria.
These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance.
It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis.
American Psychological Association (APA)
Caldeira, Roberta Lima& Jannotti-Passos, Liana Konovaloff& Carvalho, Omar dos Santos. 2017. Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae). Journal of Tropical Medicine،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1187956
Modern Language Association (MLA)
Caldeira, Roberta Lima…[et al.]. Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae). Journal of Tropical Medicine No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1187956
American Medical Association (AMA)
Caldeira, Roberta Lima& Jannotti-Passos, Liana Konovaloff& Carvalho, Omar dos Santos. Use of Molecular Methods for the Rapid Mass Detection of Schistosoma mansoni (Platyhelminthes: Trematoda) in Biomphalaria spp. (Gastropoda: Planorbidae). Journal of Tropical Medicine. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1187956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1187956