Amelioration of Inflammatory Cytokines Mix Stimulation: A Pretreatment of CD137 Signaling Study on VSMC
Joint Authors
Yan, Jinchuan
Zhong, Wei
Wang, Cuiping
Wang, Zhongqun
Shao, Chen
Li, Bo
Li, Xiao Yang
Chen, Rui
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Previous studies showed little CD137 expressed in normal vascular smooth muscle cells (VSMCs) and it is important to find a valid way to elevate it before studying its function.
The level of CD137 was detected by RT-PCR, western blot, and flow cytometry, respectively.
CD137 signaling activation was activated by agonist antibody and measured through phenotype transformation indicators and cell functions.
Proteins in supernatants were detected by ELISA.
The total CD137 elevates under different concentrations of CM treatment.
Among these, 25 ng/ml CM treatment increases the CD137 expression mostly.
However, flow cytometry demonstrates that 10 ng/ml CM elevates surface CD137 more significantly than other concentrations and reaches the peak at 36 h.
At 10 ng/ml, but not 25 ng/ml CM pretreatment, the levels of phenotype related proteins such as SM-MHC, α-SMA, and calponin decrease while vimentin and NFATc1 increase, suggesting that VSMCs undergo phenotype transformation.
Transwell, CCK-8 assay, and ELISA showed that the ability of VSMCs viability, migration, and IL-2 and IL-6 secretion induced by CD137 signaling was significantly enhanced by the pretreatment of 10 ng/ml CM.
This research suggested that 10 ng/ml CM pretreatment is more reasonable than other concentrations when exploring CD137 function in VSMCs.
American Psychological Association (APA)
Zhong, Wei& Li, Bo& Li, Xiao Yang& Wang, Zhongqun& Shao, Chen& Wang, Cuiping…[et al.]. 2017. Amelioration of Inflammatory Cytokines Mix Stimulation: A Pretreatment of CD137 Signaling Study on VSMC. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188070
Modern Language Association (MLA)
Zhong, Wei…[et al.]. Amelioration of Inflammatory Cytokines Mix Stimulation: A Pretreatment of CD137 Signaling Study on VSMC. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188070
American Medical Association (AMA)
Zhong, Wei& Li, Bo& Li, Xiao Yang& Wang, Zhongqun& Shao, Chen& Wang, Cuiping…[et al.]. Amelioration of Inflammatory Cytokines Mix Stimulation: A Pretreatment of CD137 Signaling Study on VSMC. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188070
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188070