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Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
Joint Authors
Uhlig, Stefan
Babendreyer, Aaron
Molls, Lisa
Dreymueller, Daniela
Ludwig, Andreas
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Flow conditions critically regulate endothelial cell functions in the vasculature.
Reduced shear stress resulting from disturbed blood flow can drive the development of vascular inflammatory lesions.
On endothelial cells, the transmembrane chemokine CX3CL1/fractalkine promotes vascular inflammation by functioning as a surface-expressed adhesion molecule and by becoming released as soluble chemoattractant for monocytic cells expressing the receptor CX3CR1.
Here, we report that endothelial cells from human artery, vein, or microvasculature constitutively express CX3CL1 when cultured under static conditions.
Stimulation with TNFα under static or very low shear stress conditions strongly upregulates CX3CL1 expression.
By contrast, CX3CL1 induction is profoundly reduced when cells are exposed to higher shear stress.
When endothelial cells were grown and subsequently stimulated with TNFα under low shear stress, strong adhesion of monocytic THP-1 cells to endothelial cells was observed.
This adhesion was in part mediated by transmembrane CX3CL1 as demonstrated with a neutralizing antibody.
By contrast, no CX3CL1-dependent adhesion to stimulated endothelium was observed at high shear stress.
Thus, during early stages of vascular inflammation, low shear stress typically seen at atherosclerosis-prone regions promotes the induction of endothelial CX3CL1 and monocytic cell recruitment, whereas physiological shear stress counteracts this inflammatory activation of endothelial cells.
American Psychological Association (APA)
Babendreyer, Aaron& Molls, Lisa& Dreymueller, Daniela& Uhlig, Stefan& Ludwig, Andreas. 2017. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1188088
Modern Language Association (MLA)
Babendreyer, Aaron…[et al.]. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion. Mediators of Inflammation No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1188088
American Medical Association (AMA)
Babendreyer, Aaron& Molls, Lisa& Dreymueller, Daniela& Uhlig, Stefan& Ludwig, Andreas. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1188088
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188088