Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands
Joint Authors
Chakhtoura, Marita
Sriram, Uma
Heayn, Michelle
Wonsidler, Joshua
Doyle, Christopher
Dinnall, Joudy-Ann
Gallucci, Stefania
Roberts, Rebecca A.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Sex hormones affect immune responses and might promote autoimmunity.
Endocrine disrupting chemicals such as bisphenol A (BPA) may mimic their immune effects.
Conventional dendritic cells (cDCs) are pivotal initiators of immune responses upon activation by danger signals coming from pathogens or distressed tissues through triggering of the Toll-like receptors (TLRs).
We generated in vitro murine cDCs in the absence of estrogens and measured the effects of exogenously added estrogen or BPA on their differentiation and activation by the TLR ligands LPS and CpG.
Estrogen enhanced the differentiation of GM-CSF-dependent cDCs from bone marrow precursors in vitro, and the selective estrogen receptor modulators (SERMs) tamoxifen and fulvestrant blocked these effects.
Moreover, estrogen augmented the upregulation of costimulatory molecules and proinflammatory cytokines (IL-12p70 and TNFα) upon stimulation by TLR9 ligand CpG, while the response to LPS was less estrogen-dependent.
These effects are partially explained by an estrogen-dependent regulation of TLR9 expression.
BPA did not promote cDC differentiation nor activation upon TLR stimulation.
Our results suggest that estrogen promotes immune responses by increasing DC activation, with a preferential effect on TLR9 over TLR4 stimulation, and highlight the influence of estrogens in DC cultures, while BPA does not mimic estrogen in the DC functions that we tested.
American Psychological Association (APA)
Chakhtoura, Marita& Sriram, Uma& Heayn, Michelle& Wonsidler, Joshua& Doyle, Christopher& Dinnall, Joudy-Ann…[et al.]. 2017. Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188136
Modern Language Association (MLA)
Chakhtoura, Marita…[et al.]. Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands. Mediators of Inflammation No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1188136
American Medical Association (AMA)
Chakhtoura, Marita& Sriram, Uma& Heayn, Michelle& Wonsidler, Joshua& Doyle, Christopher& Dinnall, Joudy-Ann…[et al.]. Bisphenol A Does Not Mimic Estrogen in the Promotion of the In Vitro Response of Murine Dendritic Cells to Toll-Like Receptor Ligands. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188136
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188136