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Short-Term Regulation of FcγR-Mediated Phagocytosis by TLRs in Macrophages: Participation of 5-Lipoxygenase Products
Joint Authors
Monteiro, Ana Paula T.
Pinheiro, Carla da S.
Dutra, Fabiano F.
Peters-Golden, Marc
Benjamim, Claudia F.
Canetti, Cláudio
Bozza, Marcelo T.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
TLRs recognize a broad spectrum of microorganism molecules, triggering a variety of cellular responses.
Among them, phagocytosis is a critical process for host defense.
Leukotrienes (LTs), lipid mediators produced from 5-lipoxygenase (5-LO) enzyme, increase FcγR-mediated phagocytosis.
Here, we evaluated the participation of TLR2, TLR3, TLR4, and TLR9 in FcγR-mediated phagocytosis and whether this process is modulated by LTs.
Rat alveolar macrophages (AMs), murine bone marrow-derived macrophages (BMDMs), and peritoneal macrophages (PMs) treated with TLR2, TLR3, and TLR4 agonists, but not TLR9, enhanced IgG-opsonized sheep red blood cell (IgG-sRBC) phagocytosis.
Pretreatment of AMs or BMDMs with drugs that block LT synthesis impaired the phagocytosis promoted by TLR ligands, and TLR potentiation was also abrogated in PMs and BMDMs from 5-LO−/− mice.
LTB4 production induced by IgG engagement was amplified by TLR ligands, while cys-LTs were amplified by activation of TLR2 and TLR4, but not by TLR3.
We also noted higher ERK1/2 phosphorylation in IgG-RBC-challenged cells when preincubated with TLR agonists.
Furthermore, ERK1/2 inhibition by PD98059 reduced the phagocytic activity evoked by TLR agonists.
Together, these data indicate that TLR2, TLR3, and TLR4 ligands, but not TLR9, amplify IgG-mediated phagocytosis by a mechanism which requires LT production and ERK-1/2 pathway activation.
American Psychological Association (APA)
Pinheiro, Carla da S.& Monteiro, Ana Paula T.& Dutra, Fabiano F.& Bozza, Marcelo T.& Peters-Golden, Marc& Benjamim, Claudia F.…[et al.]. 2017. Short-Term Regulation of FcγR-Mediated Phagocytosis by TLRs in Macrophages: Participation of 5-Lipoxygenase Products. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1188141
Modern Language Association (MLA)
Pinheiro, Carla da S.…[et al.]. Short-Term Regulation of FcγR-Mediated Phagocytosis by TLRs in Macrophages: Participation of 5-Lipoxygenase Products. Mediators of Inflammation No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1188141
American Medical Association (AMA)
Pinheiro, Carla da S.& Monteiro, Ana Paula T.& Dutra, Fabiano F.& Bozza, Marcelo T.& Peters-Golden, Marc& Benjamim, Claudia F.…[et al.]. Short-Term Regulation of FcγR-Mediated Phagocytosis by TLRs in Macrophages: Participation of 5-Lipoxygenase Products. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1188141
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188141