Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells
Joint Authors
Borgatti, Monica
Gambari, Roberto
Finotti, Alessia
Sacchetti, Gianni
Cabrini, Giulio
Dechecchi, Maria Cristina
Manzione, Maria Giulia
Ferrari, Davide
Spisani, Susanna
Bezzerri, Valentino
Miolo, Giorgia
Marzaro, Giovanni
Chilin, Adriana
Lampronti, Ilaria
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The angelicin analogue 4,6,4′-trimethylangelicin (TMA) was recently reported as a strong inhibitor of nuclear factor-κB (NF-κB) activity and of the expression of the interleukin-8 (IL-8) gene in bronchial epithelial cells in which the inflammatory response has been challenged with P.
aeruginosa, the most common bacterium found in the airways of patients affected by cystic fibrosis (CF).
These findings encouraged us to analyze new synthetic analogues of TMA in order to evaluate their biological activities on human bronchial epithelial CF IB3-1 cells and to find more potent anti-NF-κB agents exhibiting only minor antiproliferative effects.
Analogues able to inhibit NF-κB/DNA interaction at lower concentration than TMA were found and selected to investigate their biological activity on IB3-1 cells induced with TNF-α.
In this biological system, NF-κB-mediated IL-8 gene expression was investigated.
Some analogues showed similar activity to the lead compound TMA.
Other analogues displayed higher activities; in particular, the most interesting compounds showing relevant anti-inflammatory effects were found to cause 56–83% reduction of IL-8 mRNA expression at low concentrations (1–10 μM), without changes in cell proliferation pattern, demonstrating their potential interest for a possible development of anti-inflammatory therapy of cystic fibrosis.
American Psychological Association (APA)
Lampronti, Ilaria& Manzione, Maria Giulia& Sacchetti, Gianni& Ferrari, Davide& Spisani, Susanna& Bezzerri, Valentino…[et al.]. 2017. Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188144
Modern Language Association (MLA)
Lampronti, Ilaria…[et al.]. Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188144
American Medical Association (AMA)
Lampronti, Ilaria& Manzione, Maria Giulia& Sacchetti, Gianni& Ferrari, Davide& Spisani, Susanna& Bezzerri, Valentino…[et al.]. Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188144
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188144