Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK12AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells
Joint Authors
Guo, Zhen-Zhen
Cao, Qun-An
Liu, Li-Ping
Dai, Qiu-Yan
Wu, Hongxian
Ren, Anmin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Vagus nerve stimulation through alpha7 nicotine acetylcholine receptors (α7-nAChR) signaling had been demonstrated attenuation of inflammation.
This study aimed to determine whether PNU-282987, a selective α7-nAChR agonist, affected activities of matrix metalloproteinase (MMP) and inflammatory cytokines in nicotine-treatment RAW264.7 and MOVAS cells and to assess the underlying molecular mechanisms.
RAW264.7 and MOVAS cells were treated with nicotine at different concentrations (0, 1, 10, and 100 ng/ml) for 0–120 min.
Nicotine markedly stimulated the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun in RAW264.7 cells.
Pretreatment with U0126 significantly suppressed phosphorylation of ERK1/2 and further attenuated nicotine-induced activation of c-Jun and upregulation of MMP-2, MMP-9, monocyte chemotactic protein- (MCP-) 1, and regulated upon activation normal T cell expressed and secreted (RANTES).
Similarly, nicotine treatment also increased phosphorylation of c-Jun and expressions of MMP-2, MMP-9, MCP-1, and RANTES in MOVAS cells.
When cells were pretreated with PNU-282987, nicotine-induced activations of ERK1/2 and c-Jun in RAW264.7 cells and c-Jun in MOVAS cells were effectively inhibited.
Furthermore, nicotine-induced secretions of MMP-2, MMP-9, MCP-1, and RANTES were remarkably downregulated.
Treatment with α7-nAChR agonist inhibits nicotine-induced upregulation of MMP and inflammatory cytokines through modulating ERK1/2/AP-1 signaling in RAW264.7 cells and AP-1 in MOVAS cells, providing a new therapeutic for abdominal aortic aneurysm.
American Psychological Association (APA)
Liu, Li-Ping& Wu, Hongxian& Cao, Qun-An& Guo, Zhen-Zhen& Ren, Anmin& Dai, Qiu-Yan. 2017. Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK12AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188146
Modern Language Association (MLA)
Liu, Li-Ping…[et al.]. Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK12AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells. Mediators of Inflammation No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1188146
American Medical Association (AMA)
Liu, Li-Ping& Wu, Hongxian& Cao, Qun-An& Guo, Zhen-Zhen& Ren, Anmin& Dai, Qiu-Yan. Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK12AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1188146
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188146