Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis

Joint Authors

Long, Yang
Fan, Fang
Chen, Jiao
Tang, Shi
Jiang, Chunxia
Xu, Yong
Huang, Wei
Gao, Chenlin

Source

Mediators of Inflammation

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-01-15

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Diseases

Abstract EN

Background.

Inflammation and fibrosis are the important pathophysiologic processes in diabetic nephropathy (DN).

Maresin 1 is a potential anti-inflammatory lipid mediator, which has displayed powerful proresolving activities.

Aim.

We determine whether maresin 1 has protective effect on mouse glomerular mesangial cells (GMCs) induced by high glucose.

Methods.

We cultured GMCs stimulated by high glucose and categorized as follows: normal glucose group (5.6 mmol/L), high glucose group (30 mmol/L), mannitol group, maresin 1 intervention group (1, 10, and 100 nmol/L), maresin 1 and normal glucose group, and the N-acetylcysteine (NAC) intervention group (10 μmol/L NAC).

After 24 h, the expression of ROS, NLRP3, caspase-1, procaspase-1, IL-1β, and pro-IL-1β was detected by western-blot, RT-PCR, and immunofluorescence.

After 48 h, the expression of TGF-β1 and FN was detected by RT-PCR and ELISA.

Results.

Compared with normal glucose group, the expression of ROS, NLRP3, caspase-1, IL-1β, TGF-β1, and FN increased in high glucose group (P<0.05), but it decreased after the treatment of maresin 1 in different concentrations.

On the contrary, the expression of procaspase-1 and pro-IL-1β protein was restrained by high glucose and enhanced by maresin 1 in a dose-dependent manner (P<0.05).

Conclusion.

Maresin 1 can inhibit NLRP3 inflammasome, TGF-β1, and FN in GMCs; it may have protective effect on DN by mitigating the inflammation and early fibrosis.

American Psychological Association (APA)

Tang, Shi& Gao, Chenlin& Long, Yang& Huang, Wei& Chen, Jiao& Fan, Fang…[et al.]. 2017. Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188156

Modern Language Association (MLA)

Tang, Shi…[et al.]. Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188156

American Medical Association (AMA)

Tang, Shi& Gao, Chenlin& Long, Yang& Huang, Wei& Chen, Jiao& Fan, Fang…[et al.]. Maresin 1 Mitigates High Glucose-Induced Mouse Glomerular Mesangial Cell Injury by Inhibiting Inflammation and Fibrosis. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188156

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1188156