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Indirubin Treatment of Lipopolysaccharide-Induced Mastitis in a Mouse Model and Activity in Mouse Mammary Epithelial Cells
Joint Authors
Hu, Chang-Min
Chen, Yingyu
Lai, Jin-lun
Liu, Yu-hui
Peng, Yong-chong
Ge, Pan
He, Chen-fei
Liu, Chang
Guo, Ai-zhen
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Indirubin is a Chinese medicine extracted from indigo and known to be effective for treating chronic myelogenous leukemia, neoplasia, and inflammatory disease.
This study evaluated the in vivo anti-inflammatory activity of indirubin in a lipopolysaccharide- (LPS-) induced mouse mastitis model.
The indirubin mechanism and targets were evaluated in vitro in mouse mammary epithelial cells.
In the mouse model, indirubin significantly attenuated the severity of inflammatory lesions, edema, inflammatory hyperemia, milk stasis and local tissue necrosis, and neutrophil infiltration.
Indirubin significantly decreased myeloperoxidase activity and downregulated the production of tumor necrosis factor-α, interleukin-1β (IL-1β), and IL-6 caused by LPS.
In vitro, indirubin inhibited LPS-stimulated expression of proinflammatory cytokines in a dose-dependent manner.
It also downregulated LPS-induced toll-like receptor 4 (TLR4) expression and inhibited phosphorylation of LPS-induced nuclear transcription factor-kappa B (NF-κB) P65 protein and inhibitor of kappa B.
In addition to its effect on the NF-κB signaling pathway, indirubin suppressed the mitogen-activated protein kinase (MAPK) signaling by inhibiting phosphorylation of extracellular signal-regulated kinase (ERK), P38, and c-jun NH2-terminal kinase (JNK).
Indirubin improved LPS-induced mouse mastitis by suppressing TLR4 and downstream NF-κB and MAPK pathway inflammatory signals and might be a potential treatment of mastitis and other inflammatory diseases.
American Psychological Association (APA)
Lai, Jin-lun& Liu, Yu-hui& Peng, Yong-chong& Ge, Pan& He, Chen-fei& Liu, Chang…[et al.]. 2017. Indirubin Treatment of Lipopolysaccharide-Induced Mastitis in a Mouse Model and Activity in Mouse Mammary Epithelial Cells. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188225
Modern Language Association (MLA)
Lai, Jin-lun…[et al.]. Indirubin Treatment of Lipopolysaccharide-Induced Mastitis in a Mouse Model and Activity in Mouse Mammary Epithelial Cells. Mediators of Inflammation No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1188225
American Medical Association (AMA)
Lai, Jin-lun& Liu, Yu-hui& Peng, Yong-chong& Ge, Pan& He, Chen-fei& Liu, Chang…[et al.]. Indirubin Treatment of Lipopolysaccharide-Induced Mastitis in a Mouse Model and Activity in Mouse Mammary Epithelial Cells. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1188225
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188225