A Role of the ABCC4 Gene Polymorphism in Airway Inflammation of Asthmatics
Joint Authors
Kim, Seung-Hyun
Palikhe, Sailesh
Uuganbayar, Udval
Trinh, Hoang Kim Tu
Ban, Ga-Young
Yang, Eun-Mi
Park, Hae-Sim
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The ATP-binding cassette subfamily C member 4 gene encodes a transmembrane protein involved in the export of proinflammatory molecules, including leukotriene, prostaglandin, and sphingosine-1-phosphate across the plasma membrane.
Those metabolites play important roles in asthma.
We investigated the potential associations between ABCC4 gene polymorphisms and asthma phenotype.
In total, 270 asthma patients and 120 normal healthy controls were enrolled for a genetic association study.
Two polymorphisms (−1508A>G and −642C>G) in the ABCC4 promoter were genotyped.
The functional variability of the promoter polymorphisms was analyzed by luciferase reporter assay.
Inflammatory cytokine levels were measured by enzyme-linked immunosorbent assay.
Serum and urinary eicosanoid metabolites, sphingosine-1-phosphate, were evaluated by quadrupole time-of-flight mass spectrometry.
Asthma patients carrying the G allele at −1508A>G had significantly higher serum levels of periostin, myeloperoxidase, and urinary levels of 15-hydroxyeicosatetraenoic acid and sphingosine-1-phosphate (P=0.016, P=0.027, P=0.032, and P=0.010, resp.) compared with noncarrier asthma patients.
Luciferase activity was significantly enhanced in human epithelial A549 cells harboring a construct containing the −1508G allele (P<0.01 for each) compared with a construct containing the −1508A allele.
A functional polymorphism in the ABCC4 promoter, −1508A>G, may increase extracellular 15-hydroxyeicosatetraenoic acid, sphingosine-1-phosphate, and periostin levels, contributing to airway inflammation in asthmatics.
American Psychological Association (APA)
Palikhe, Sailesh& Uuganbayar, Udval& Trinh, Hoang Kim Tu& Ban, Ga-Young& Yang, Eun-Mi& Park, Hae-Sim…[et al.]. 2017. A Role of the ABCC4 Gene Polymorphism in Airway Inflammation of Asthmatics. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1188266
Modern Language Association (MLA)
Palikhe, Sailesh…[et al.]. A Role of the ABCC4 Gene Polymorphism in Airway Inflammation of Asthmatics. Mediators of Inflammation No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1188266
American Medical Association (AMA)
Palikhe, Sailesh& Uuganbayar, Udval& Trinh, Hoang Kim Tu& Ban, Ga-Young& Yang, Eun-Mi& Park, Hae-Sim…[et al.]. A Role of the ABCC4 Gene Polymorphism in Airway Inflammation of Asthmatics. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1188266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188266