Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MSMS and Comet Assay
Joint Authors
Erdem, Onur
Tona, Alessandro
Reipa, Vytas
Atha, Donald H.
Coskun, Erdem
Nelson, Bryant C.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
To evaluate methods for analysis of genotoxic effects on mammalian cell lines, we tested the effect of three common genotoxic agents on Chinese hamster ovary (CHO) cells by single-cell gel electrophoresis (comet assay) and gas chromatography-tandem mass spectrometry (GC-MS/MS).
Suspension-grown CHO cells were separately incubated with etoposide, bleomycin, and ethyl methanesulfonate and analyzed by an alkaline comet assay and GC-MS/MS.
Although DNA strand breaks were detected by the comet assay after treatment with all three agents, GC-MS/MS could only detect DNA nucleobase lesions oxidatively induced by bleomycin.
This demonstrates that although GC-MS/MS has limitations in detection of genotoxic effects, it can be used for selected chemical genotoxins that contribute to oxidizing processes.
The comet assay, used in combination with GC-MS/MS, can be a more useful approach to screen a wide range of chemical genotoxins as well as to monitor other DNA-damaging factors.
American Psychological Association (APA)
Atha, Donald H.& Coskun, Erdem& Erdem, Onur& Tona, Alessandro& Reipa, Vytas& Nelson, Bryant C.. 2020. Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MSMS and Comet Assay. Journal of Nucleic Acids،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1188300
Modern Language Association (MLA)
Atha, Donald H.…[et al.]. Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MSMS and Comet Assay. Journal of Nucleic Acids No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1188300
American Medical Association (AMA)
Atha, Donald H.& Coskun, Erdem& Erdem, Onur& Tona, Alessandro& Reipa, Vytas& Nelson, Bryant C.. Genotoxic Effects of Etoposide, Bleomycin, and Ethyl Methanesulfonate on Cultured CHO Cells: Analysis by GC-MSMS and Comet Assay. Journal of Nucleic Acids. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1188300
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188300