Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages
Joint Authors
Cho, Chang-Won
Jang, Mi
Yang, Deok Chun
Ahn, Sungeun
Lim, Tae-Gyu
Hong, Hee-Do
Rhee, Young Kyoung
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
We recently reported the immune-enhancing effects of a high-molecular-weight fraction (HMF) of CW in macrophages and immunosuppressed mice, and this effect was attributed to a crude polysaccharide.
As polysaccharides may also have anti-inflammatory functions, we investigated the anti-inflammatory effects and related molecular mechanisms of a crude polysaccharide (HMFO) obtained from HMF of CW in mice with dextran sulfate sodium- (DSS-) induced colitis and in lipopolysaccharide-induced RAW 264.7 macrophages.
HMFO ameliorated the pathological characteristics of colitis and significantly reduced production of proinflammatory cytokines in the serum.
Histological analysis indicated that HMFO improved the signs of histological damage such as abnormal crypts, crypt loss, and inflammatory cell infiltration induced by DSS.
In addition, HMFO inhibited iNOS and COX-2 protein expression, as well as phosphorylated NF-κB p65 levels in the colon tissue of mice with DSS-induced colitis.
In macrophages, HMFO inhibited several cytokines and enzymes involved in inflammation such as prostaglandin E2, nitric oxide, tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2 by attenuating nuclear factor-κB (NF-κB) and mitogen-activated protein kinases.
HMFO attenuated inflammation both in vitro and in vivo, primarily by inhibiting NF-κB activation.
Our findings indicate that HMFO is a promising remedy for treating inflammatory bowel diseases, such as colitis.
American Psychological Association (APA)
Cho, Chang-Won& Ahn, Sungeun& Lim, Tae-Gyu& Hong, Hee-Do& Rhee, Young Kyoung& Yang, Deok Chun…[et al.]. 2017. Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1188303
Modern Language Association (MLA)
Cho, Chang-Won…[et al.]. Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages. Mediators of Inflammation No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1188303
American Medical Association (AMA)
Cho, Chang-Won& Ahn, Sungeun& Lim, Tae-Gyu& Hong, Hee-Do& Rhee, Young Kyoung& Yang, Deok Chun…[et al.]. Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1188303
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188303