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Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis
Joint Authors
García-Laorden, M. Isabel
Stroo, Ingrid
Terpstra, Sanne
Florquin, Sandrine
Medema, Jan Paul
van´t Veer, Cornelis
de Vos, Alex F.
van der Poll, Tom
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Escherichia (E.) coli is the most common causative pathogen in peritonitis, the second most common cause of sepsis.
Granzymes (gzms) are serine proteases traditionally implicated in cytotoxicity and, more recently, in the inflammatory response.
We here sought to investigate the role of gzms in the host response to E.
coli-induced peritonitis and sepsis in vivo.
For this purpose, we used a murine model of E.
coli intraperitoneal infection, resembling the clinical condition commonly associated with septic peritonitis by this bacterium, in wild-type and gzmA-deficient (gzmA−/−), gzmB−/−, and gzmAxB−/−mice.
GzmA and gzmB were predominantly expressed by natural killer cells, and during abdominal sepsis, the percentage of these cells expressing gzms in peritoneal lavage fluid decreased, while the amount of expression in the gzm+ cells increased.
Deficiency of gzmA and/or gzmB was associated with increased bacterial loads, especially in the case of gzmB at the primary site of infection at late stage sepsis.
While gzm deficiency did not impact neutrophil recruitment into the abdominal cavity, it was accompanied by enhanced nucleosome release at the primary site of infection, earlier hepatic necrosis, and more renal dysfunction.
These results suggest that gzms influence bacterial growth and the host inflammatory response during abdominal sepsis caused by E.
coli.
American Psychological Association (APA)
García-Laorden, M. Isabel& Stroo, Ingrid& Terpstra, Sanne& Florquin, Sandrine& Medema, Jan Paul& van´t Veer, Cornelis…[et al.]. 2017. Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188317
Modern Language Association (MLA)
García-Laorden, M. Isabel…[et al.]. Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188317
American Medical Association (AMA)
García-Laorden, M. Isabel& Stroo, Ingrid& Terpstra, Sanne& Florquin, Sandrine& Medema, Jan Paul& van´t Veer, Cornelis…[et al.]. Expression and Function of Granzymes A and B in Escherichia coli Peritonitis and Sepsis. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188317
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188317