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Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis
Joint Authors
Toffoletto, Barbara
Curcio, Francesco
Domenis, Rossana
Zanutel, Rossella
Caponnetto, Federica
Cifù, Adriana
Pistis, Cinzia
Di Benedetto, Paolo
Causero, Araldo
Pozzi, Massimo
Bassini, Fabrizio
Niazi, Kayvan R.
Soon-Shiong, Patrick
Fabris, Martina
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The purpose of this study is to characterize synovial fluid- (SF-) derived exosomes of patients with gonarthrosis comparing two methods of isolation and to investigate their immune regulatory properties.
Extracellular vesicles (EVs) have been isolated from inflamed SF by polymer precipitation method and quantified by Exocet kit and by nanoparticle tracking analysis.
Vesicles expressed all the specific exosomal markers by immunoblot and FACS.
After isolation with Exoquick, a relevant contamination by immune complexes was detected, which required further magnetic bead-based purification to remove.
SF-derived exosomes significantly stimulated the release of several inflammatory cytokines and chemokines and metalloproteinases by M1 macrophages but did not influence the expression of CD80 and CD86 costimulatory molecules.
In conclusion, we characterized purified exosomes isolated from inflamed SF and demonstrate that purified exosomes are functionally active in their ability to stimulate the release of proinflammatory factors from M1 macrophages.
Our data indicate that SF-derived exosomes from gonarthrosis patients play a role in disease progression.
American Psychological Association (APA)
Domenis, Rossana& Zanutel, Rossella& Caponnetto, Federica& Toffoletto, Barbara& Cifù, Adriana& Pistis, Cinzia…[et al.]. 2017. Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188355
Modern Language Association (MLA)
Domenis, Rossana…[et al.]. Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188355
American Medical Association (AMA)
Domenis, Rossana& Zanutel, Rossella& Caponnetto, Federica& Toffoletto, Barbara& Cifù, Adriana& Pistis, Cinzia…[et al.]. Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188355