Mitofusin 2 Promotes Apoptosis of CD4+ T Cells by Inhibiting Autophagy in Sepsis
Joint Authors
Dong, Ning
Yao, Yong-Ming
Lu, Zhongqiu
Ying, Lan
Zhao, Guang-Ju
Wu, You
Ke, He-Liang
Hong, Guang-Liang
Zhang, Hui
Wu, Yao
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-19
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Apoptosis of CD4+ T cells is a primary pathophysiological mechanism of immune dysfunction in the pathogenesis of sepsis.
Mitofusin 2 (Mfn2), an integral mitochondrial outer membrane protein, has been confirmed to be associated with cellular metabolism, proliferation, and apoptosis.
The function of Mfn2 in CD4+ T cell apoptosis in sepsis is poorly understood.
Here, we discovered increased in vivo Mfn2 expression, autophagy deficiency, and elevated cell apoptosis in murine splenic CD4+ T cells after cecal ligation and puncture (CLP).
We also observed almost identical results in splenic CD4+ T cells upon lipopolysaccharide (LPS) stimulation in vitro.
Furthermore, overexpression of Mfn2 resulted in impaired autophagy and increased apoptosis in Jurkat cells.
Pharmacological inhibition of autophagy with 3-methyladenine enhanced Mfn2 overexpression-induced cell apoptosis.
In addition, overexpression of Mfn2 downregulated phorbol myristate acetate (PMA)/ionomycin-, rapamycin- and starvation-induced autophagy in Jurkat T cells.
Taken together, these data indicate a critical role of Mfn2 in CD4+ T cell apoptosis in sepsis and the underlying mechanism of autophagy deficiency.
American Psychological Association (APA)
Ying, Lan& Zhao, Guang-Ju& Wu, You& Ke, He-Liang& Hong, Guang-Liang& Zhang, Hui…[et al.]. 2017. Mitofusin 2 Promotes Apoptosis of CD4+ T Cells by Inhibiting Autophagy in Sepsis. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1188364
Modern Language Association (MLA)
Ying, Lan…[et al.]. Mitofusin 2 Promotes Apoptosis of CD4+ T Cells by Inhibiting Autophagy in Sepsis. Mediators of Inflammation No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1188364
American Medical Association (AMA)
Ying, Lan& Zhao, Guang-Ju& Wu, You& Ke, He-Liang& Hong, Guang-Liang& Zhang, Hui…[et al.]. Mitofusin 2 Promotes Apoptosis of CD4+ T Cells by Inhibiting Autophagy in Sepsis. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1188364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188364