Printability Optimization of Gelatin-Alginate Bioinks by Cellulose Nanofiber Modification for Potential Meniscus Bioprinting
Joint Authors
Wang, Chenyu
Luo, Wenbin
Song, Zhengyi
Wang, Zhonghan
Wang, Zhenguo
Li, Zuhao
Liu, He
Liu, Qingping
Wang, Jincheng
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-05
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Meniscal injury is more likely to cause a permanent alteration of the biomechanical and biological environment of the knee joint, mainly due to the morphological mismatch and substantial loss of meniscal tissues.
Herein, to overcome this challenge, we developed an improved bioink with enhanced printability, while maintaining the biocompatibility of major cellular component of the meniscus, namely fibrochondrocytes.
Firstly, cellulose nanofiber (CNF) was mixed with gelatin-alginate thermal-responsive bioinks to improve the printability.
Afterward, individual-specific meniscal prototypes based on the 3D reconstruction of MRI data were bioprinted using our bioink.
The rheological and printability properties of the bioinks were characterized to select proper bioink content and bioprinting parameters.
And then, a series of biological characterizations of the bioprinted samples, such as cell viability, metabolic activity, and extracellular matrix accumulation, were carried out in vitro.
The results indicated that superior rheological performance and printability of CNF-modified bioink were achieved, ensuring high-precision bioprinting of specific-designed meniscal prototype when compared with the non-CNF-containing counterparts.
Meanwhile, biological tests indicated that fibrochondrocytes encapsulated within the CNF-modified bioink maintained long-term cellular viability as well as acceptable extracellular matrix accumulation.
This study demonstrates that the CNF-modified bioink is in favor of the printing fidelity of specific meniscus by improved rheological properties, minimizing the mismatch between artificial meniscal implants and native knee joint tissues, thereby permitting the evolution of clinical therapeutic methods of meniscal reconstruction.
American Psychological Association (APA)
Luo, Wenbin& Song, Zhengyi& Wang, Zhonghan& Wang, Zhenguo& Li, Zuhao& Wang, Chenyu…[et al.]. 2020. Printability Optimization of Gelatin-Alginate Bioinks by Cellulose Nanofiber Modification for Potential Meniscus Bioprinting. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1188401
Modern Language Association (MLA)
Luo, Wenbin…[et al.]. Printability Optimization of Gelatin-Alginate Bioinks by Cellulose Nanofiber Modification for Potential Meniscus Bioprinting. Journal of Nanomaterials No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1188401
American Medical Association (AMA)
Luo, Wenbin& Song, Zhengyi& Wang, Zhonghan& Wang, Zhenguo& Li, Zuhao& Wang, Chenyu…[et al.]. Printability Optimization of Gelatin-Alginate Bioinks by Cellulose Nanofiber Modification for Potential Meniscus Bioprinting. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1188401
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188401