Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
Joint Authors
Cemazar, Maja
Sersa, Gregor
Signori, Emanuela
Lampreht Tratar, Ursa
Loiacono, Luisa
Kamensek, Urska
Fazio, Vito Michele
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cancer immunotherapy is currently one of the leading approaches in cancer treatment.
Gene electrotransfer of plasmids encoding interleukin 12 (IL-12) into the cells leads to the production of IL-12, which drives immune cell polarization to an antitumoral response.
One of the cell types that shows great promise in targeting tumor cells under the influence of IL-12 cytokine milieu is that of macrophages.
Therefore, the aim of this study was to evaluate gene electrotransfer of antibiotic resistance-free plasmid DNA-encoding murine IL-12 (mIL-12) in mice bearing aggressive B16F10 murine melanoma.
IL-12 electrotransfer resulted in the complete long-term eradication of the tumors.
Serum mIL-12 and murine interferon γ (mIFNγ) were increased after IL-12 gene electrotransfer.
Further on, hematoxylin and eosin (HE) staining showed increased infiltration of immune cells that lasted from day 4 until day 14.
Immunohistochemistry (IHC) staining of F4/80, MHCII, and CD11c showed higher positive staining in the IL-12 gene electrotransfer group than in the control groups.
Immune cell infiltration into the tumors and the high density of MHCII- and CD11c-positive cells suggest an antitumor polarization of macrophages and the presence of antigen-presenting cells that contributes to the important antitumor effectiveness of IL-12.
American Psychological Association (APA)
Lampreht Tratar, Ursa& Loiacono, Luisa& Cemazar, Maja& Kamensek, Urska& Fazio, Vito Michele& Sersa, Gregor…[et al.]. 2017. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma. Mediators of Inflammation،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188427
Modern Language Association (MLA)
Lampreht Tratar, Ursa…[et al.]. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma. Mediators of Inflammation No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1188427
American Medical Association (AMA)
Lampreht Tratar, Ursa& Loiacono, Luisa& Cemazar, Maja& Kamensek, Urska& Fazio, Vito Michele& Sersa, Gregor…[et al.]. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma. Mediators of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1188427
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188427