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Sequential Delivery of BMP2-Derived Peptide P24 by Thiolated ChitosanCalcium Carbonate Composite Microspheres Scaffolds for Bone Regeneration
Joint Authors
Wang, Zhaozhen
Liu, Xujie
Martin, Vidmi Taolam
Abdi, Mohamed Abdullahi
Chen, Lijun
Gong, Yong
Yan, Yiran
Song, Liming
Liu, Zhongxun
Zhang, Xianliao
Yu, Bo
Chen, Yan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The combination of tissue-engineered bone scaffolds with osteogenic induction molecules is an important strategy for critical-sized bone defects repair.
We synthesized a novel thiolated chitosan/calcium carbonate composite microsphere (TCS-P24/CA) scaffold as a carrier for bone morphogenetic protein 2- (BMP2-) derived peptide P24 and evaluated the release kinetics of P24.
The effect of TCS-P24/CA scaffolds on the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) was evaluated by scanning electron microscope (SEM), CCK-8, ALP assay, alizarin red staining, and PCR.
A 5 mm diameter calvarial defect was created, then new bone formation was evaluated by Micro-CT and histological examination at 4 and 8 weeks after operation.
We found the sequential release of P24 could last for 29 days.
Meanwhile, BMSCs revealed spindle-shaped surface morphology, indicating the TCS-P24/CA scaffolds could support cell adhesion and mRNA levels for ALP, Runx2, and COL1a1 were significantly upregulated on TCS-10%P24/CA scaffold compared with other groups in vitro (p<0.05).
Similarly, the BMSCs exhibited a higher ALP activity as well as calcium deposition level on TCS-10%P24/CA scaffolds compared with other groups (p<0.05).
Analysis of in vivo bone formation showed that the TCS-10%P24/CA scaffold induced more bone formation than TCS-5%P24/CA, TCS/CA, and control groups.
This study demonstrates that the novel TCS-P24/CA scaffolds might contribute to the delivery of BMP2-derived Peptide P24 and is considered to be a potential candidate for repairing bone defects.
American Psychological Association (APA)
Wang, Zhaozhen& Liu, Xujie& Martin, Vidmi Taolam& Abdi, Mohamed Abdullahi& Chen, Lijun& Gong, Yong…[et al.]. 2020. Sequential Delivery of BMP2-Derived Peptide P24 by Thiolated ChitosanCalcium Carbonate Composite Microspheres Scaffolds for Bone Regeneration. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1188447
Modern Language Association (MLA)
Wang, Zhaozhen…[et al.]. Sequential Delivery of BMP2-Derived Peptide P24 by Thiolated ChitosanCalcium Carbonate Composite Microspheres Scaffolds for Bone Regeneration. Journal of Nanomaterials No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1188447
American Medical Association (AMA)
Wang, Zhaozhen& Liu, Xujie& Martin, Vidmi Taolam& Abdi, Mohamed Abdullahi& Chen, Lijun& Gong, Yong…[et al.]. Sequential Delivery of BMP2-Derived Peptide P24 by Thiolated ChitosanCalcium Carbonate Composite Microspheres Scaffolds for Bone Regeneration. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1188447
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188447