A Combination Therapy of pHRE-Egr1-HSV-TKAnti-CD133McAb-131IMFH Mediated by FePt Nanoparticles for Liver Cancer Stem Cells
Joint Authors
Lin, Mei
Shi, Yujuan
Guo, Ting
Xiao, Yanhong
Zhang, Jun
Jiang, Xingmao
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-12
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
It has been evidenced that liver cancer stem cells (LCSCs) are to blame hepatocellular carcinoma (HCC) occurrence, development, metastasis, and recurrence.
Using iron-platinum nanoparticles (FePt-NPs) as a carrier and CD133 antigen as a target, a new strategy to targetly kill LCSCs by integrating HSV-TK suicide gene, 131I nuclide irradiation, and magnetic fluid hyperthermia (MFH) together was designed and investigated in the present study.
The results showed that FePt-NPs modified with PEI (PEI-FePt-NPs) could bind with DNA, and the best binding ratio was 1 : 40 (mass ratio).
Moreover, DNA binding to PEI-FePt-NPs could refrain from Dnase1 enzyme digestion and could release under certain conditions.
LCSCs (CD133+ Huh-7 cells) were transfected with pHRE-Egr1-HSV-TK by PEI-FePt-NPs, and the transfection efficiency was 53.65±3.40%.
These data showed a good potential of PEI-FePt-NPs as a gene transfer carrier.131I was labeled with anti-CD133McAb in order to facilitate therapy targeting.
The combined intervention of pHRE-Egr1-HSV-TK/anti-CD133McAb-131I/MFH mediated by PEI-FePt-NPs could greatly inhibit LCSCs’ growth and induce cell apoptosis in vitro, significantly higher than any of the individual interventions (p<0.05).
This study offers a practicable idea for LCSC treatment, and PEI-FePt-NPs may act as novel nonviral gene vectors and a magnetic induction medium.
American Psychological Association (APA)
Lin, Mei& Xiao, Yanhong& Jiang, Xingmao& Zhang, Jun& Guo, Ting& Shi, Yujuan. 2020. A Combination Therapy of pHRE-Egr1-HSV-TKAnti-CD133McAb-131IMFH Mediated by FePt Nanoparticles for Liver Cancer Stem Cells. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1188542
Modern Language Association (MLA)
Lin, Mei…[et al.]. A Combination Therapy of pHRE-Egr1-HSV-TKAnti-CD133McAb-131IMFH Mediated by FePt Nanoparticles for Liver Cancer Stem Cells. Journal of Nanomaterials No. 2020 (2020), pp.1-15.
https://search.emarefa.net/detail/BIM-1188542
American Medical Association (AMA)
Lin, Mei& Xiao, Yanhong& Jiang, Xingmao& Zhang, Jun& Guo, Ting& Shi, Yujuan. A Combination Therapy of pHRE-Egr1-HSV-TKAnti-CD133McAb-131IMFH Mediated by FePt Nanoparticles for Liver Cancer Stem Cells. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-15.
https://search.emarefa.net/detail/BIM-1188542
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188542