Nanocoatings of Bovine Serum Albumin on Glass: Effects of pH and Temperature
Joint Authors
Acuña-Nelson, Sergio-Miguel
Bastías-Montes, José-Miguel
Cerda-Leal, Fabiola-Rossana
Parra-Flores, Julio-Enrique
Aguirre-García, Juan-Salvador
Toledo, Pedro G.
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Protein adsorption is influenced by many factors such as temperature, pH, protein size and structure, or surface energy and roughness, among others.
Self-assembled monolayers (SAMs) and the Langmuir-Blodgett (LB) technique are two of the techniques more used to produces ultrathin films of proteins on surfaces.
In this work, we established protocols for the preparation of nanocoatings of bovine serum albumin (BSA) protein on glass surface using SAMs and LB.
Furthermore, we determined how small changes in temperature and pH can affect the covering when SAMs are used.
Using a combination of different analyses, such as relative roughness, dynamic contact angles, and atomic force microscopy (AFM), it was possible to establish conditions to obtain a uniform nanocoating using SAMs.
The results of the analysis of the nanocoating performed using the LB technique were very similar to those obtained using SAMs.
The Derjaguin–Landau–Verwey–Overbeek (DLVO) theory in conjunction with the AFM images showed that electrostatic interactions are very important in the self-assembly process, but a process dominated solely by attraction is not sufficient to achieve a good SAM nanocoating, since it does not allow proper orientation and packaging of BSA molecules on the glass surface.
American Psychological Association (APA)
Acuña-Nelson, Sergio-Miguel& Bastías-Montes, José-Miguel& Cerda-Leal, Fabiola-Rossana& Parra-Flores, Julio-Enrique& Aguirre-García, Juan-Salvador& Toledo, Pedro G.. 2020. Nanocoatings of Bovine Serum Albumin on Glass: Effects of pH and Temperature. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1188585
Modern Language Association (MLA)
Acuña-Nelson, Sergio-Miguel…[et al.]. Nanocoatings of Bovine Serum Albumin on Glass: Effects of pH and Temperature. Journal of Nanomaterials No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1188585
American Medical Association (AMA)
Acuña-Nelson, Sergio-Miguel& Bastías-Montes, José-Miguel& Cerda-Leal, Fabiola-Rossana& Parra-Flores, Julio-Enrique& Aguirre-García, Juan-Salvador& Toledo, Pedro G.. Nanocoatings of Bovine Serum Albumin on Glass: Effects of pH and Temperature. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1188585
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1188585