Evaluation of Osteogenic Potentials of Titanium Dioxide Nanoparticles with Different Sizes and Shapes

Joint Authors

Wang, Jianbo
Ren, Yixing
Feng, Xinxing
Lang, Xinhua
Du, Zhipo
Niu, Xufeng

Source

Journal of Nanomaterials

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-11-11

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Chemistry
Civil Engineering

Abstract EN

TiO2 nanoparticles (NPs) have the potential to be used in the human body as an artificial implant because of their special physicochemical properties.

However, information about the effects of TiO2 NPs on preosteoblast proliferation and osteogenic differentiation is not clear.

In this work, we focus on the impact of TiO2 NPs with different shapes and sizes on the proliferation and differentiation of MC3T3-E1 cells.

The morphology and physicochemical properties of TiO2 NPs are analyzed by scanning electron microscopy, transmission electron microscopy, Quadrasorb SI analyzer, dynamic light scattering, and zeta potential.

The MTT results indicate that when the concentration of TiO2 NPs is less than 20 μg/mL, the proliferation of osteoblasts is preserved the most.

The expression of alkaline phosphatase and osteocalcin is detected by BCA and enzyme-linked immunosorbent assay to analyze the differentiation of osteoblasts.

The results indicate that both rutile and anatase TiO2 NPs have a significant inhibiting influence on the differentiation of osteoblasts.

Alizarin Red staining is performed on cells to detect the mineralized nodules.

The results show that TiO2 NPs can promote the mineralization of MC3T3-E1 cells.

Then, we study the oxidative stress response of MC3T3-E1 cells by flow cytometry analysis, and all TiO2 NPs induce the excessive generation of reactive oxide species.

On the other hand, our study also shows that the early apoptotic cells increase significantly.

TiO2 NPs are swallowed by cells, and then the agglomerated particles enter mitochondria, causing the shape of mitochondria to change and vacuolation to appear.

All these results show that TiO2 NPs have certain cytotoxicity to cells, but they also promote the mineralization and maturation of osteoblasts.

American Psychological Association (APA)

Ren, Yixing& Feng, Xinxing& Lang, Xinhua& Wang, Jianbo& Du, Zhipo& Niu, Xufeng. 2020. Evaluation of Osteogenic Potentials of Titanium Dioxide Nanoparticles with Different Sizes and Shapes. Journal of Nanomaterials،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1188602

Modern Language Association (MLA)

Ren, Yixing…[et al.]. Evaluation of Osteogenic Potentials of Titanium Dioxide Nanoparticles with Different Sizes and Shapes. Journal of Nanomaterials No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1188602

American Medical Association (AMA)

Ren, Yixing& Feng, Xinxing& Lang, Xinhua& Wang, Jianbo& Du, Zhipo& Niu, Xufeng. Evaluation of Osteogenic Potentials of Titanium Dioxide Nanoparticles with Different Sizes and Shapes. Journal of Nanomaterials. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1188602

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1188602